Proteomic Serum Profile of Fatigued Men Receiving Localized External Beam Radiation Therapy for Non-Metastatic Prostate Cancer  Nada Lukkahatai, PhD, RN, Sajni Patel, MS, Marjan Gucek, PhD, Chao-Pin Hsiao, PhD, RN, Leorey N. Saligan, PhD, RN, CRNP  Journal of Pain and Symptom Management  Volume 47, Issue 4, Pages 748-756.e4 (April 2014) DOI: 10.1016/j.jpainsymman.2013.05.016 Copyright © 2014 Terms and Conditions

Fig. 1 Sypro ruby stained gel image. The Sypro ruby stained gel image illustrates numbered positions of peptides, which were identified by LC-MS/MS to detect proteins that consistently changed in the 2-D DIGE image of depleted sera collected from baseline to midpoint of EBRT. These numbers were used to identify the proteins that were detected in the gel spot. LC-MS/MS=liquid chromatography tandem mass spectrometry; 2-D DIGE=dimensional difference gel electrophoresis; EBRT=external beam radiation therapy; pI= isoelectric point; MW= molecular weight. Journal of Pain and Symptom Management 2014 47, 748-756.e4DOI: (10.1016/j.jpainsymman.2013.05.016) Copyright © 2014 Terms and Conditions

Fig. 2 Protein identification of gel spots. These proteins were found to consistently change in the 2-D DIGE image of the sera of four HF subjects collected at baseline and midpoint of external beam radiation therapy. FC= fold change; HF=high fatigue; 2-D DIGE=two-dimensional difference gel electrophoresis. Journal of Pain and Symptom Management 2014 47, 748-756.e4DOI: (10.1016/j.jpainsymman.2013.05.016) Copyright © 2014 Terms and Conditions

Fig. 3 Western Blot band intensities. Non-normalized Western blot band intensities confirming the three proteins observed to consistently change from the 2-D DIGE image of the sera of 12 subjects collected at baseline (Day 0) and midpoint (Day 21) of external beam radiation therapy. HF=high fatigue; NF=no fatigue; 2-D DIGE=two-dimensional difference gel electrophoresis. Journal of Pain and Symptom Management 2014 47, 748-756.e4DOI: (10.1016/j.jpainsymman.2013.05.016) Copyright © 2014 Terms and Conditions