Microslots and Microfluidic Logic Yael Maguire and Manu Prakash Oct 12, 2006

, Sweedler/Whitesides et al, APL, 1997 If we make life... how will we understand what we’ve made? Sweedler et al, Chem Rev, 1999 , Sweedler/Whitesides et al, APL, 1997 starting to create life in the lab from scratch using biomolecules or proteins form=function analyze Walton et al, AnalChem, 2003 Massin et al, JMR, 2003 1013-1014 (100pmols -1nmol) biomolecules

8x more sensitive than best planar probe 160 um plug 8x more sensitive than best planar probe 1014 molecules

Future 1010 molecules at room temperature 100 sensors in 5mm2

Complexity in fluid manipulation 2005 2006 2007 1960’s Moore’s Law for Microfluidics 1960’s saw the birth of high Reynolds number fluidic logic, an all-fluidic logic family dependent on inertial properties of fluid jets. Complex machine controllers, displays and preliminary information processing units were built, competing 60’s electronics. Thus a highly complex labyrinth of macro- channels could manipulate multiple fluid jets precisely. Complexity in integrated fluid manipulation has increase manifold since than with introduction of large, bulky robots (used in every biotech lab) and more recently microfluidics. Modern times have seen a a rebirth of complex fluid manipulation with the introduction of microfluidic large scale integration and elastomeric pneumatic valves. It is now possible to fit 10,000 valves on a single 2cmx2cm chip controlling femtoliters of fluid. These chips require external control with 100’s of macro-sized solenoids and immense reagent to chip plumbing. Increasing Complexity

Microfluidic Bubble Logic We propose a new way of on-chip internal process control in microfluidic devices using Bubble Logic. We demonstrate all-fluidic computation at low Reynolds number Bubble is a bit, carrying both information and materials payload Integration of Chemistry and Computation (process control) Here we show AND/OR logic gates, toggle flip-flop and a Ring oscillator. Droplet based segmented-flow reactor : No dispersion Rapid mixing KHz range operation No external control elements Non-linear residence time Nonlinearity Bistability Cascadability Feedback