Week 6 Team has split into Dry Lab and Wet Lab Dry Lab: Wet Lab:

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Presentation transcript:

Week 6 Team has split into Dry Lab and Wet Lab Dry Lab: Wet Lab: Erika, - James, Prudence, -Chris, Clinton, -Tom, Yanis, -Krupa,

Wet Lab Two key tasks: DNA synthesis Protocols and Reagents

DNA Synthesis Genes Terminators Promoter and RBS

Promoters RBS Promoter and RBS Inducible Constitutive 1.p43 Sigma B 1.Phyper-spank 2.pXyl Constitutive 1.p43 2.pVeg Sigma B 1.pctc 2.pgsiB RBS =12 combinations 1.gsiB 2.spoVG

Genes Genes Signal Peptide Biomaterials Registry Light+Motility 1.YtvA 2.EspE Signal Peptide 1.SacB 2.LipA Biomaterials 1.Elastin 2.EAK 16 Registry 1.Antibiotics 2.GFPmut3b =4 Combinations

Insertion Sequence Terminators Terminators Registry Insertion Sequence 1.Double Terminator Insertion Sequence 1.PyrD 2.AmyE

Protocols Cloning, Culturing, Transformation, Testing,

Cloning

Culturing and Transformation This week we are planning to test culturing and transformation using integration vector. If successful will linearise vector and test for linear integration

Have defined testing Constructs. This week need to work out testing protocols, Microscope training on Thursday!

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