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Dnyanasadhana College, Thane. Satish Pradhan Dnyanasadhana College, Thane. Department of Chemistry T.Y.B.Sc. Analytical Chemistry Paper-IV Sem-VI Size Exclusion Chromatography By Dr.Bhagure G.R. 11/16/2018

Contents 3.3 Size Exclusion Chromatography (SEC) (02L) 3.3.1Introduction, Basic Principle, Applications of SEC 11/16/2018

Size Exclusion Chromatography This separation method originated in 1959 at the Biochemical Institute in Uppsala, Sweden. Size Exclusion Chromatography Gel filtration Gel permeation 11/16/2018

Separation of molecules according to Molecular size Used for separation of proteins, polymeres, sugars in different type of fruit Separation of molecules according to Molecular size Large size molecule Small size molecule Introduction 11/16/2018

ex; agar, agarose,polyacryamide cross linked dextrin's Basic Principles of Size Exclusion Chromatography Stationary Phase Gel molecules ex; agar, agarose,polyacryamide cross linked dextrin's Sample molecules 11/16/2018

Column Packing 2. Gel Size 5 to 10 um 1. Polymer beads 11/16/2018

Applications of Size exclusion chromatography : 1 Separation of High molecular weight compounds ex. Proteins can be separated from amino acids 2 Homologue and oligomers can be separated from each other 3 Determination of molecular weight or distribution of the molecular weight 4 Desalting is a another applications which involves removal of salts and low molecular weight compound from macromolecules 11/16/2018

Advantages of Size exclusion chromatography : 1 Method is very simple to perform 2 It provides conditions for maximum stability to very labile materials such as enzymes. 3 Gels can be reused 4 Useful in various industries like petroleum ,coating and varnishes and in the field of medicine 11/16/2018

Thank You For Kind Attention 11/16/2018