In vivo reduction or blockade of interleukin-1β in primary osteoarthritis influences expression of mediators implicated in pathogenesis K.S. Santangelo,

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In vivo reduction or blockade of interleukin-1β in primary osteoarthritis influences expression of mediators implicated in pathogenesis K.S. Santangelo, G.J. Nuovo, A.L. Bertone Osteoarthritis and Cartilage Volume 20, Issue 12, Pages 1610-1618 (December 2012) DOI: 10.1016/j.joca.2012.08.011 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 In vivo detection of the CMV promoter (A) in 1 μg starting DNA and expression of reporter genes for AAV5 (B) and adenoviral (Ad) vectors (C) were confirmed using PCR, qRT-PCR, and Xenogen IVIS System, respectively. DNA containing the CMV promoter was not detected (ND) in patellar cartilage collected from group 2 Ad-injected animals at either 120 or 180 days of age. A significant difference for this promoter between groups 1 and 3 was not present at either harvest date. Statistical differences in GFP expression were not noted in cartilage at 120 or 180 days of age in groups 1 and 3, as determined using one-way ANOVA followed by pairwise comparisons using Tukey's 95% confidence intervals. Luciferase (Luc) expression was detected in all Ad-Luc injected knees immediately prior to harvest on both collection ages (C). TV = AAV5-shRNA-TV; NTV = AAV5-shRNA-NTV; Ad-Luc, vector containing a CMV-driven coding region for firefly luciferase (Luc); Ad-hIRAP, vector containing a CMV-driven coding region for hIRAP. Osteoarthritis and Cartilage 2012 20, 1610-1618DOI: (10.1016/j.joca.2012.08.011) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Median (range provided) IHC scoring (A) and OA grading (C), mean body weight (D), and representative photomicrographs (B) (100, final magnification), of medial tibial plateaus from treatment groups harvested at specified ages. Animals were injected with viral vectors (N = 4 animals per treatment group per collection date) at 60 days of age and harvested at either 120 or 180 days of age. At time of treatment, animals were assigned to one of two groups to investigate reduction or blockade, respectively, of IL-1β vs respective vector controls: TV or NTV were injected into contralateral knees (group 1); Ad-Luc or Ad-hIRAP were injected into contralateral knees (group 2). A third set of animals (group 3) received TV or PBS in contralateral knees to challenge the efficacy of TV in the absence of vector control. A final set of animals (group 4) did not receive injections in either knee to allow reference of the above treatment groups to untreated controls. The percentage of chondrocytes staining positive for IL-1β were assigned a score of 0–5 and a scale of 1–3 was used to gauge the intensity of immunostaining (total ranging from 0–8). Histological grading of serial coronal sections of the medial weight-bearing compartment of each knee was performed using adapted Mankin criteria based upon characteristic features of OA in this species. The score for each structure ranged from 0 (normal) to 14 (severe structural damage and complete loss of toluidine blue staining), providing a total chondropathy index ranging from 0–28. Data were analyzed using the ANOVA, Kruskal–Wallis test, followed by Dunn's multiple comparison post-hoc test. (Please see Fig. 1 for full description of abbreviations. †P < 0.05.) Osteoarthritis and Cartilage 2012 20, 1610-1618DOI: (10.1016/j.joca.2012.08.011) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions