Figure 2 Representative MSI data obtained from human joints

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Figure 2 Representative MSI data obtained from human joints Figure 2 | Representative MSI data obtained from human joints. Peptides of 1,431.8 m/z, corresponding to fibronectin, are detected and visualized by mass spectrometry imaging (MSI) in tissue sections from human cartilage and synovium. Considerable differences in the abundance of fibronectin can be observed in healthy and osteoarthritic (OA) tissues. These data can be confirmed by immunohistochemistry (IHC) analysis of consecutive sections from the same samples using anti-fibronectin antibodies. Scale bars: 200 µm in IHC images; 500 µm in MSI images. Permissions obtained from Wiley © Cillero-Pastor, B. et al. Arthritis Rheum. 65, 710–720 (2013)42, and from Springer © Cillero-Pastor, B. et al. Protein classification and distribution in osteoarthritic human synovial tissue by matrix-assisted laser desorption ionization mass spectrometry imaging. Anal. Bioanal. Chem. 407, 2213–2222 (2015)64. Permissions obtained from Wiley © Cillero-Pastor, B. et al. Arthritis Rheum. 65, 710–720 (2013), and from Springer © Cillero- Pastor, B. et al. Protein classification and distribution in osteoarthritic human synovial tissue by matrix-assisted laser desorption ionization mass spectrometry imaging. Anal. Bioanal. Chem. 407, 2213–2222 (2015). Rocha, B. et al. (2016) Mass spectrometry imaging: a novel technology in rheumatology Nat. Rev. Rheumatol. doi:10.1038/nrrheum.2016.184