Analysis of the morphological dynamics of blastocysts after vitrification/warming: defining new predictive variables of implantation Aila Coello, Ph.D.,

Slides:

Advertisements

Similar presentations

Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice Zaraq Khan, M.B.B.S., Heather S.

Advertisements

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

International Committee for Monitoring Assisted Reproductive Technology (ICMART) world report: assisted reproductive technology 2003 Karl G. Nygren,

Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (≥10 cells), bringing into question current embryological.

Demián Glujovsky, M.D., M.Sc., Cynthia Farquhar, M.D., M.P.H.

Study of nucleation status in the second cell cycle of human embryo and its impact on implantation rate Jesus Aguilar, Ph.D., Irene Rubio, Ph.D., Elkin.

International Committee for Monitoring Assisted Reproductive Technology: world report on assisted reproductive technology, 2005 Fernando Zegers-Hochschild,

Adenomyosis does not affect implantation, but is associated with miscarriage in patients undergoing oocyte donation José A. Martínez-Conejero, Ph.D.,

The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer Yimin Shu, M.D., Ph.D., Jill Watt, Ph.D., Janice.

Christine Jablonski, M. D. , Marco Alifano, M. D

Patient and cycle characteristics predicting high pregnancy rates with single-embryo transfer: an analysis of the Society for Assisted Reproductive Technology.

Infertility evaluation and treatment among women in the United States

Fernando Zegers-Hochschild, M.D. Fertility and Sterility

José Bellver, M. D. , Marco A. B. Melo, M. D. , Ernesto Bosch, M. D

Michael M. Alper, M.D. Fertility and Sterility

Composition of single-step media used for human embryo culture

Clinical application of oocyte vitrification: a systematic review and meta-analysis of randomized controlled trials Ana Cobo, Ph.D., César Diaz, M.D.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Mohamad Irani, M. D. , David Reichman, M. D. , Alex Robles, M. D

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos Alberto Tejera, Ph.D., Javier Herrero, Ph.D.,

Inmaculada Molina, Ph. D. , Juan V. Martínez, M. S. , José F

Outcome of cryotransfer of embryos developed from vitrified oocytes: double vitrification has no impact on delivery rates Ana Cobo, Ph.D., Damià Castellò,

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies Francisco Dominguez, Ph.D., Marcos Meseguer,

Sarina Martini, B. A. , Bradley J. Van Voorhis, M. D. , Barbara J

Iris G. Insogna, M. D. , Malinda S. Lee, M. D. , M. B. A. , Rebecca M

Grant D.E. McWilliams, D.O., John L. Frattarelli, M.D.

Ahmad Majzoub, M. D. , Nicholas N. Tadros, M. D. , A

The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility.

Oocyte cryopreservation

Two interesting cases of ovarian pregnancy after in vitro fertilization–embryo transfer and its successful laparoscopic management Selvaraj Priya, M.D.,

Lena Dain, M. D. , David Bider, M. D. , Jacob Levron, M. D

Martin Ivec, B.Sc., Borut Kovacic, Ph.D., Veljko Vlaisavljevic, Ph.D.

Sperm DNA fragmentation levels in testicular sperm samples from azoospermic males as assessed by the sperm chromatin dispersion (SCD) test Marcos Meseguer,

Six years' experience in ovum donation using vitrified oocytes: report of cumulative outcomes, impact of storage time, and development of a predictive.

Time-lapse deselection model for human day 3 in vitro fertilization embryos: the combination of qualitative and quantitative measures of embryo growth

Automatic time-lapse instrument is superior to single-point morphology observation for selecting viable embryos: retrospective study in oocyte donation

Association between prior appendectomy and/or tonsillectomy in women and subsequent pregnancy rate: a cohort study Li Wei, Ph.D., Thomas MacDonald, M.D.,

Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential Jason E. Swain, Ph.D., Doug Carrell, Ph.D.,

Type of chromosome abnormality affects embryo morphology dynamics

Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality Shuoping Zhang,

Morphokinetic analysis and embryonic prediction for blastocyst formation through an integrated time-lapse system Yamileth Motato, Ph.D., María José de.

Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,

Clomiphene citrate at 50: the dawning of assisted reproduction

Obesity reduces uterine receptivity: clinical experience from 9,587 first cycles of ovum donation with normal weight donors José Bellver, M.D., Antonio.

Beata Seeber, M. D. , M. S. C. E. , Mary D. Sammel, Sc. D

Should embryos developing to blastocysts on day 7 be cryopreserved and transferred: an analysis of pregnancy and implantation rates George Kovalevsky,

Introduction Fertility and Sterility

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Ana Cobo, Ph. D. , Aila Coello, Ph. D. , Jose Remohí, M. D

Storage of human oocytes in the vapor phase of nitrogen

José Bellver, M. D. , Yanira Ayllón, M. D. , Marcos Ferrando, M. D

Website quality assessment: Mistaking apples for oranges

Effects of early cleavage patterns of human embryos on subsequent in vitro development and implantation Yamato Mizobe, M.S., Naoto Oya, M.S., Reiko Iwakiri,

Sperm selection by swim-up in terms of deoxyribonucleic acid fragmentation as measured by the sperm chromatin dispersion test is altered in heavy smokers

Amy Criniti, M. D. , Angela Thyer, M. D. , Gregory Chow, M. D

Fertility and Sterility: an evaluation

Impact of subclinical hypothyroidism in women with recurrent early pregnancy loss Lia A. Bernardi, M.D., Ronald N. Cohen, M.D., Mary D. Stephenson, M.D.,

International Committee for Monitoring Assisted Reproductive Technologies: World Report on Assisted Reproductive Technologies, 2007 Osamu Ishihara, M.D.,

Martha Luna, M. D. , Marlena Duke, M. Sc. , Alan Copperman, M. D

Genetic evaluation procedures at sperm banks in the United States

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Parabolic trend in endometrial thickness at embryo transfer in in vitro fertilization/intracytoplasmic sperm injection cases with clinical pregnancy evidence

In vitro sildenafil citrate use as a sperm motility stimulant

Treatment of heterotopic cervical pregnancy after in vitro fertilization–embryo transfer by using transvaginal ultrasound–guided aspiration and instillation.

Ongoing pregnancy rates in intrauterine insemination are affected by late follicular- phase progesterone levels Antonio Requena, M.D., María Cruz, Ph.D.,

Contact among families who share the same sperm donor

Outcomes of vitrified early cleavage-stage and blastocyst-stage embryos in a cryopreservation program: evaluation of 3,150 warming cycles Ana Cobo, Ph.D.,

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Presentation transcript:

Analysis of the morphological dynamics of blastocysts after vitrification/warming: defining new predictive variables of implantation Aila Coello, Ph.D., Marcos Meseguer, Ph.D., Arancha Galán, Ph.D., Lucia Alegre, Ph.D., José Remohí, M.D., Ana Cobo, Ph.D. Fertility and Sterility Volume 108, Issue 4, Pages 659-666.e4 (October 2017) DOI: 10.1016/j.fertnstert.2017.07.1157 Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Percentage of implanting blastocysts with morphological dynamics variables within or beyond the ranges defined by the quartile limits for the total data set. The four panels show ranges and implantation for (A) initial ZP thickness, *P=.02; (B) minimum ZP thickness, *P=.03; (C) initial blastocyst area, *P=.04; and (D) maximum blastocyst area, *P=.004. Fertility and Sterility 2017 108, 659-666.e4DOI: (10.1016/j.fertnstert.2017.07.1157) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Asociación Española de Biología de la Reproducción (ASEBIR) blastocyst classification based on expansion degree, ICM, and TE quality. Fertility and Sterility 2017 108, 659-666.e4DOI: (10.1016/j.fertnstert.2017.07.1157) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Drawing tools using EmbryoViewer for assessment of a warmed blastocyst. (A) Blastocyst area and ZP thickness measures immediately after warming. (B) Blastocyst area and ZP thickness measures before transfer. (C) The ICM area measure. Fertility and Sterility 2017 108, 659-666.e4DOI: (10.1016/j.fertnstert.2017.07.1157) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 Bar graphics for the initial and minimum ZP thicknesses and the initial and maximum blastocyst areas. Fertility and Sterility 2017 108, 659-666.e4DOI: (10.1016/j.fertnstert.2017.07.1157) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 4 Blastocyst reexpansion. (A) Classification of the KID blastocysts according to blastocoele reexpansion. (B) Implantation rate of the KID blastocysts according to blastocoele reexpansion. (C) Proportion of blastocysts according to ASEBIR classification previtrification. (D) Proportion of blastocysts according to degree of expansion previtrification. Fertility and Sterility 2017 108, 659-666.e4DOI: (10.1016/j.fertnstert.2017.07.1157) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions