UV laser fluorophore options Fortessa HCI 5 Laser FACSAria Cell Sorter

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UV laser fluorophore options Fortessa HCI 5 Laser FACSAria Cell Sorter

BUV dyes BUV 395 Trying to figure out a complicated 10-18 color phenotyping panel? You should really consider one of the BUV dyes. BUV 395 CD4 stained PBMC These dyes also have very little spillover into any other detectors BUV 496 BUV 496 CD4 stained PBMC

Fixable Blue viability dye ThermoFisher Blue Viability dye intensity in two UV detectors -Works in both UV detectors -I would not recommend also trying another BUV dye at the same time Compensation from blue viability dye into violet laser detectors -”spills” into BV421 more than BV510 -Overall very manageable Conclusion- Blue Viability Dye is a nice alternative to DAPI or other viability stains in crowded multicolor panels.

DAPI and BV421 DAPI alone Background: DAPI has a very broad excitation range. Before the addition of the UV laser, DAPI was collected in the BV421 channel off the violet laser. Summary: Now you can detect DAPI off the UV laser and also simultaneously detect a BV421 antibody off the violet laser. DAPI plus CD4 BV421 ”live” gate including BV421 CD4+ cells