Microscope 8/22/17.

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Presentation transcript:

Microscope 8/22/17

Head/Body The upper part of the microscope; houses the optical parts

Base Located at the bottom; supports the microscope; houses the illuminator

Arm Middle section; Connects to the base and supports the head

Eyepiece (Ocular)  Know both names The part you look through

Eyepiece Tube Holds the eyepiece in place

nosepiece Houses the objectives; mounted on a rotating wheel to change to another objective.

Objective Lenses Magnifies the specimen There are typically 3 different objective lenses (x4, x10, x40, or x100) Located on the nosepiece

Coarse Adjustment Knob Moves the stage up and down to help you get the specimen into view

Fine Adjustment Knob Moves the stage slightly to help you sharpen or “fine” tune your view of the specimen

Stage Where the specimen is placed

Stage Clips Holds the slides in place so they do not move around

Aperture The hole in the stage where the light passes through

Illuminator Light source of the microscope; usually a typical lightbulb

Diaphragm Controls the amount of light that can pass through the stage

How to prep a slide

Step 1: Prep The Slide Place the slide on a flat surface. Add one drop of water to the slide. Add Specimen to the drop.

Step 2: Add the CoverSlip Carefully place the the coverslip onto of the water droplet. Do this slowly so that you do not get air bubbles underneath your coverslip. If there are bubbles, CAREFULLY press down on the coverslip to remove the bubbles.

How to Use A Microscope

Step 1: Double Check your Microscope is prepared for use. Make sure that the stage is as far from the objective lenses as possible. Rotate the nosepiece so that the lowest strength of objective lens is selected. The diaphragm is all the way open. Cord is plugged in!

Step 2: Situate the Slide Move the stage clips to the side. Place the slide directly over the aperture. (MAKE SURE THE SPECIMEN PART IS OVER THE APERTURE.) Once slide is placed move the stage clips securing the slide to the stage.

Step 3: Adjust to Focus Using the coarse and fine adjustment knobs to focus the specimen. BEFORE YOU SWITCH TO A STRONGER OBJECTIVE LENS ALWAYS MAKE SURE THAT THE STAGE IS LOWERED ALL THE WAY.