Catalase Concentrations Olivia Clements Makenzie Nelson Alexis Beyke Lily

Experiment An experiment was conducted to determine how the concentration of catalase affects a solution of H2O2. One trial was run on each of the three different concentrations of catalase. A logger pro was used to measure the pressure of the oxygen given off by the reaction. Hypothesis: If the concentration of catalase affects the reaction rate, then the highest concentration of catalase (800 units/mL) will have a higher reaction rate than the lower concentrations (200 units/mL, 400 units/mL).

Materials Needed 200 units/mL catalase Lab Journal Distilled Water 800 units/mL catalase Sterilized Droppers 3 sterilized test tubes 3% Hydrogen Peroxide (H2O2) 5 sterilized beakers Safety Equipment (Goggles, Gloves, Aprons, etc.) Logger Pro™ Computer

Steps of the Experiment Add 3mL of H2O2 to each of the three test tubes, then add 5mL of distilled water to each of those three test tubes. Add 2 drops of 200 units/mL catalase into test tube 1. Quickly cap the test tube with the measuring stopper and lightly agitate the solution while measuring the reaction rate using Logger Pro™ on the computer for 100 seconds. Record Results in lab journal. Add 2 drops of 400 units/mL catalase into test tube 2. Repeat step 3 and record results in lab journal. Add 2 drops of 800 units/mL catalase into test tube 3.

Results Our results showed that the 800 units/mL concentration of catalase had a steeper slope, and therefore, a greater reaction rate, than the 200 and 400 units/mL solutions.

Citations Chemistry for Biologists. (2009). Enzymes. [Accessed January 13, 2016]. Retrieved from http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm Project Lead The Way. (2014). Project 3.2.4 Investigating Enzyme Action. [Accessed January 12, 2016]. Retrieved from https://pltw.instructure.com/courses/227763/assignments/4365430