Howard H. Kim, Michael Funaro, Svetlana Mazel, Marc Goldstein, Peter N

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Flow cytometric characterization of apoptosis and chromatin damage in spermatozoa  Howard H. Kim, Michael Funaro, Svetlana Mazel, Marc Goldstein, Peter N. Schlegel, Darius A. Paduch  Reproductive BioMedicine Online  Volume 26, Issue 4, Pages 393-395 (April 2013) DOI: 10.1016/j.rbmo.2012.12.005 Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 FACS analysis of human spermatozoa stained with different fluorescent markers. (A) With progression of the apoptosis signal, membrane is permeable to annexin V and PI; thus both signals increase in intensity. (B) AV is a late marker of apoptosis as spermatozoa with PF-1 fluorescence values between 0–104 show no change in AV. (C) Typical fluorogram of PF-1 against PI shows broad range of PF-1 fluorescent intensity for PI negative spermatozoa; this indicates that some PI-negative spermatozoa are PF-1-positive, allowing early detection of apoptotic spermatozoa. (D) DiIC1(5) drops in intensity as PF-1 increases; in PF-1-negative spermatozoa DiIC1(5) has highest level of intensity (103–104). DiIC – DiIC1(5) stain; PF-1=proprietary stain; PI=propidium iodide, Annexin V-PE - AV conjugated to phycoerythrin. Reproductive BioMedicine Online 2013 26, 393-395DOI: (10.1016/j.rbmo.2012.12.005) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

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