Bacterial Isolation & Identification

Slides:

Advertisements

Similar presentations

Upper and Lower Respiratory Tract Infection

Advertisements

●BIOCHEMOCAL UNKNOWN ●OXIDATION/ FERMENTATION ●CATALASE ●oXIDASE

STREAKING AN AGAR PLATE FOR ISOLATIONOF BACTERIAL COLONIES BACTERIAL COLONIES.

Host parasite interactions. Table 28-1 Table 28-2.

Lab equipment & procedures Chapter Ⅱ. Microscopes A. Light Microscopes 1. Single Lens 2. Compound microscopes eye pieceobjectiveTotal M Low P10X10X100X.

Laboratory 7 & 8 Bacteriology. Bacteria Small Unicellular Organisms Can be grown in nutrient enriched environments (Agar, Broth) Standard Medias: Tryptic.

Comparison of the Diagnostic Value of the Standard Tube Agglutination Test and the ELISA IgG and IgM in Patients with Brucellosis Presented by Dr. Md.

Pseudomonas - Microscopic appearance - Cultural characteristics

CHAPTER 3. Content purification of cultures morphological and pureculture studies biochemical tests.

Pathogenic Microorganisms. Bacteria  Fungi  Parasites.

Haemophilus - Microscopic appearance - Serological methods for detection of H.influenzae - Identification of Haemophilus species - Cultural characteristics.

Lab 23 Respiratory Microorganisms. Objectives Interpret hemolysis results Classify various Streptococcus species.

Sample Comprehensive Laboratory exam

Identification of Strep and Staph and Isolating gram – pathogens Lab # 10 Medgar Evers College Prof. Santos.

Identification of Pathogenic Bacteria by Laboratory Methods M. Kent Froberg, MD.

Ex. 15 Oxidase Test Objectives: ?? Cytochrome oxidases help with transfer of electrons from cytochromes to oxygen.

Comparison of the diagnostic value of STA test and ELISA IgG and IgM in patients with Brucellosis Mustafa Ertek, Halil Yzgi, Zulal Ozkart et al. Turk.

Case 1 Proteus mirabilis Image A Gram stain slide A 70-year-old man presents with lower back and groin pain with dysuria for a week. He also complains.

Comparison of the diagnostic value of STA test and ELISA IgG and IgM in patients with Brucellosis Mustafa Ertek, Halil Yzgi, Zulal Ozkart et al. Turk J.

Lab 10- Colony isolation Mixed culture and unknown.

Hematopoietic and Lymphatic System. Blood Parasites.

(Wounds, Abscesses, Burns, Sinuses)

Urinary Tract Infection Department of Microbiology

Components Preparation Inoculation

Things to Do Today Observe Unknown gm (-) results Start Urinalysis Start gm (+) ID –Check for hemolysis –Catalase test –Coagulase test –Direct stain colonies.

Pathogenic Microorganisms. Bacteria  Fungi  Parasites.

Identification of unknown bacterium

Ear culture D. M. M. Lab..

Detection, Prophylaxis and Treatment of Bacterial Infection.

Biochemical Activities of Microorganisms Part (1)

Austin Stutts 41111/26/13.   1. Identification of Staphylococcus saprophyticus in lab (Genus)  2. Identification of Staphylococcus saprophyticus in.

Experiment 6 Isolation & identification of pathogenic cocci

*5 I’s Inoculation – Implanting Microbes into or upon culture media.

Laboratory Diagnosis Chapter 8. APPROACH TO LABORATORY DIAGNOSIS ● The laboratory diagnosis of infectious diseases involves two main approaches, the bacteriologic.

Isolation of microorganisms

Urinary Tract Infection Department of Microbiology

WHOOPING COUGH Nasal discharge, Paroxysmal Respiratory distress, Pneumonia.

Microbiology lab Urinary system. Urine culture steps 1.Collect the sample in sterile container: 1.Midstream catch. 2.Through a catheter. 3.Suprapubic.

Biochemical tests MSc. Sarah Ahmed

Proteus-Providencia-Morganella group

Sample collection & laboratory methods for identification bacteria

Culturing Bacteria Growth Media

Chapter 41 Culture Techniques

Identification of Unknown Bacteria: individual assignment

Urinary Tract Infection Department of Microbiology

GIT-block Microbiology Lab.

Identification of Unknown Bacteria: individual assignment

Aerobic Non-Spore Forming Gram-Positive Bacilli

Order: Pseudomonadales

Ear culture D. M. M. Lab..

Pure Culture Techniques

Survey of Microbial Diseases

Pasteurella multocida

Jane L. Burns, Jean-Marc Rolain Journal of Cystic Fibrosis

Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Vocabulary Coliforms Pure culture Types of culture.

Corynebacterium spp Anaerobic Gram positive bacilli,

Processing of clinical specimen

Ex. 10: Streak Plate Method of Isolation

Bsc (Hons) Biomedical Sciences, UKM

Ear Culture بسم الله الرحمن الرحيم

Enterobacteriaceae Clinical Bacteriology II CLS 413

II- Streptococci Characters of Streptococci Gram positive cocci

A novel chromogenic medium for isolation of Pseudomonas aeruginosa from the sputa of cystic fibrosis patients Larissa Laine, John D. Perry, Jenner Lee,

Streptococcus pneumoniae

Cerebrospinal fluid Culture

Streaking an Agar Plate For

Cerebrospinal fluid Culture

Practical No.17 Proteus & Pseudomonas.

Ear Culture بسم الله الرحمن الرحيم

Presentation transcript:

Bacterial Isolation & Identification sample/ swab Day 1 BA McA streak Blood agar Mac Conkey agar 37 0C 18-20 hr

pick up a suspected colony Day 2 subculture BA McA subculture 37 0C 18-20 hr

BA McA Day 3 pure culture Staining: shape, Gram+, Gram- Primary test: OF, catalase, oxidase, motility 37 0C 18-20 hr

compare the result with the table genus identification Day 4 Secondary test 37 0C 18-20 hr

compare the result with the table bacterial identification (genus and species) Pseudomonas aeruginosa Day 5 Sensitivity test

compare the result to the manufacturer report Day 6