DNA Clean-Up Using MagNA Beads

Library Prep Using the “With-Bead” Method

Blunt-end repair Shearing the genomic DNA Adapter ligation Fill-in Pre-hybridization PCR “with-beads”

Shearing the genomic DNA

Blunt-end repair

Blunt-end repair

Adapter ligation

Adapter ligation IS7 INLINE INDEX DNA INLINE INDEX IS8

Adapter ligation

Fill-in

Fill-in

Pre-hybridization PCR “with-beads”

Gene capture for target sequencing

Hybridization Bind to beads and wash Post-hybridization indexing PCR

Gene capture

Add 3 µL of the mixture to each of the empty tubes labeled Target enrichment – capture with sample name, and then add 7 µL of “PreH” sample (adjust according to conc., usually 100ng ~ 500ng in total) to each tube

IS7 INLINE INDEX DNA INLINE INDEX IS8

HYB Baits Mix（19µL） Lib Mix（10µL） HYB Baits Mix Lib Mix（10µL） 95 °C 65 °C 65 °C 60 °C 55 °C 50 °C 50 °C 5 min 5 min 300 min 300 min 300 min 300 min forever

Bind to beads and wash Please don’t stop the pcr program, we will still use it. Open the hybridization machine before you do the experiment.

Bind to beads and wash

Bind to beads and wash

Post-hybridization indexing PCR

Adapter ligation P5 IS7 INLINE INDEX DNA INLINE INDEX IS8 P7