Russell S. Traister, MD, PhD, Crystal E. Uvalle, BS, Gregory A

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Presentation transcript:

Phenotypic and genotypic association of epithelial IL1RL1 to human TH2-like asthma Russell S. Traister, MD, PhD, Crystal E. Uvalle, BS, Gregory A. Hawkins, PhD, Deborah A. Meyers, PhD, Eugene R. Bleecker, MD, Sally E. Wenzel, MD Journal of Allergy and Clinical Immunology Volume 135, Issue 1, Pages 92-99.e10 (January 2015) DOI: 10.1016/j.jaci.2014.06.023 Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 qRT-PCR of epithelial ST2L mRNA expression by asthma severity. RNA was extracted from fresh epithelial brushings, and cDNA was prepared and quantified by qRT-PCR, with ST2L expression normalized to GAPDH. Data represented as geometric mean ± SE of the mean. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Epithelial ST2L protein expression in endobronchial biopsies by immunohistochemistry. Tissue biopsies were stained with anti-ST2 antibody, with representative images shown for healthy controls (A), participants with mild/moderate asthma (B), and those with severe asthma (C). Quantification of all staining is also shown (D). Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Epithelial ST2L mRNA expression by TH2-like score. TH2-like score was calculated, as described in the text, based on median splits of Feno and epithelial CLCA1 and eotaxin-3 expression and elevated eosinophils, using a cutoff of 300 eosinophils/mm3. Data are represented as a geometric mean ± 95% CI. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Regulation of sST2 mRNA (A) and protein (B) levels by IFN-γ in air-liquid interface culture of epithelial cells. Cells were stimulated with 10 ng/mL of IFN-γ for 8 days. sST2 mRNA levels were determined by qRT-PCR. sST2 protein levels were determined by ELISA. Each symbol represents an independent experiment. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Outline of study design and analysis steps. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 sST2 BAL fluid levels by asthma severity. sST2 levels were determined by ELISA. Data are represented as quantile plots. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 qRT-PCR of BAL IFN-γ mRNA levels by asthma severity. RNA was extracted from the cellular fraction of BAL, and cDNA was prepared and quantified by qRT-PCR, with IFN-γ mRNA expression normalized to GAPDH. Data are represented as quantile plots. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Regulation of ST2L mRNA levels by IFN-γ in ALI culture of epithelial cells. Cells were stimulated with 10 ng/mL of IFN-γ for 8 days. ST2L mRNA levels were determined by qRT-PCR. Each symbol represents an independent experiment. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E5 Regulation of sST2 mRNA (A) and protein (B) levels by IL-13 in ALI culture of epithelial cells. Cells were stimulated with 10 ng/mL of IL-13γ for 8 days. sST2 mRNA levels determined by qRT-PCR. sST2 protein levels determined by ELISA. Each symbol represents an independent experiment. Journal of Allergy and Clinical Immunology 2015 135, 92-99.e10DOI: (10.1016/j.jaci.2014.06.023) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions