OSMOSIS & DIFFUSION LAB

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OSMOSIS & DIFFUSION LAB Animation from: http://www.lionden.com/cell_animations.htm

SAFETY DO NOT EAT OR DRINK ANYTHING IN LAB! IODINE IS POISONOUS WEAR AN APRON! IODINE WILL STAIN YOUR SKIN AND CLOTHING Image from: http://www.llnsciencepark.be/en2/images/caution.jpg

IODINE TEST for STARCH Iodine turns BLUE-BLACK in the presence of starch. http://faculty.ntcc.edu/mhearron/cell_chemistry.htm

STATION #1 1. Fill cup with ¾ full with water 2. Go to Station #2 http://www.chemistryland.com/CHM107Lab/Lab5/Soil/Lab5Exp3Soil.html 1. Fill cup with ¾ full with water 2. Go to Station #2

STATION #2 Add 30 drops of IODINE to water in your cup. Be careful! Image by Riedell Add 30 drops of IODINE to water in your cup. Be careful! IODINE IS POISONOUS! It will stain your clothes! It will stain your skin! 2. Use a permanent marker to label the level of liquid in the cup. 3. Go to Station #3.

STATION #3 1. Hold membrane bag UNDER RUNNING WATER and rub it http://www.beartoes.com/images/sink.jpg 1. Hold membrane bag UNDER RUNNING WATER and rub it with your fingers until it opens. 2. Run water THROUGH the INSIDE of the bag. 3. Tie a KNOT at ONE end of the bag. 4. Go to Station #4.

STATION #4 1. Make sure you have tied a knot in ONE end of Image by Riedell 1. Make sure you have tied a knot in ONE end of the membrane bag. 2. Fill bag with STARCH (use 2 fingers to determine how much to fill it) 3. Pinch end closed and tie a knot in other end. Leave a little space at the end. 4. Go to Station #5.

STATION #5 RINSE the membrane bag ………really, really, really, really, really, really, really, really, (I mean it!) REALLLLLLLLY WELL. 2. Go to Station #6.

STATION #6 1. DRY OFF the membrane bag and use a BALANCE to determine its MASS. Write this down in your lab packet Remember which balance you use so you can use the SAME ONE tomorrow!

STATION #7 1. Use PERMANENT MARKER to mark level of liquid in your membrane tube. 2. Take cup back to your table. 3. START EXPERIMENT by placing the membrane bag in the cup. 4. WAIT AND WATCH WHAT HAPPENS! Image by Riedell