Virginia TrueAllele® Validation Study: Casework Comparison

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Presentation transcript:

Virginia TrueAllele® Validation Study: Casework Comparison American Academy of Forensic Sciences February, 2013 Washington, DC Mark W Perlin, PhD, MD, PhD Kiersten Dormer, MS and Jennifer Hornyak, MS Cybergenetics, Pittsburgh, PA Lisa Schiermeier-Wood, MS and Susan Greenspoon, PhD Department of Forensic Science, Richmond, VA Cybergenetics © 2003-2013 Cybergenetics © 2007-2012

Case Composition • 72 criminal cases • 92 evidence items • 111 genotype comparisons Criminal offense • 18 homicide • 12 robbery • 6 sexual assault • 20 weapon

DNA Mixture Distribution

DNA Mixture Data Quantitative peak heights at a locus peak size peak

Data Summary – “Alleles” Over threshold, peaks are labeled as allele events Allele Pair 7, 7 7, 10 7, 12 7, 14 10, 10 10%10, 12 10, 14 12, 12 12, 14 14, 14 All-or-none allele peaks, each given equal status Threshold

CPI Information 2.26 CPI 6.70 Nothing reported 25

SWGDAM 2010 Guidelines Higher threshold for human review Allele Pair 7, 7 7, 10 7, 12 7, 14 10, 10 0%10, 12 10, 14 12, 12 12, 14 14, 14 Under threshold, alleles less used Threshold

Modified CPI Information 2.26 56 CPI mCPI 1.75 2.12 6.70 Nothing reported 25

TrueAllele® Casework ViewStation User Client Database Server Interpret/Match Expansion Visual User Interface VUIer™ Software Parallel Processing Computers

Validated Genotyping Method Perlin MW, Sinelnikov A. An information gap in DNA evidence interpretation. PLoS ONE. 2009;4(12):e8327. Perlin MW, Legler MM, Spencer CE, Smith JL, Allan WP, Belrose JL, Duceman BW. Validating TrueAllele® DNA mixture interpretation. Journal of Forensic Sciences. 2011;56(6):1430-47. Perlin MW, Belrose JL, Duceman BW. New York State TrueAllele® Casework validation study. Journal of Forensic Sciences. 2013;58(6):in press.

Separate mixture data into two contributor components Mixture Weight Separate mixture data into two contributor components 25% 75%

Genotype Thorough: consider every possible genotype solution Objective: does not know the comparison genotype Allele Pair 7, 7 7, 10 7, 12 7, 14 10, 10 98%10, 12 10, 14 12, 12 12, 14 14, 14 Victim's allele pair Explain the peak pattern Another person's allele pair Better explanation has a higher likelihood

TrueAllele® Sensitivity 2.26 56 CPI mCPI TrueAllele 1.75 5.52 2.12 6.70 10.93 Nothing reported 25 9

TrueAllele® Specificity True exclusions, without false inclusions – 19.69

TrueAllele® Reproducibility Concordance in two independent computer runs standard deviation (within-group) 0.305 log(LR2) log(LR1)

TrueAllele® Virginia Outcomes 144 cases analyzed 72 case reports 9 trials City Court Charge Sentence Richmond Federal Weapon 50 years Alexandria Bank robbery 90 years Quantico Military Rape 3 years Chesapeake State Robbery 26 years Arlington Molestation 22 years Homicide 35 years Fairfax Abduction 33 years Norfolk 8 years Charlottesville 15 years

TrueAllele computer genotyping is more effective than human review Conclusions TrueAllele® Casework DNA mixture interpretation is: A reliable method • sensitive • specific • reproducible TrueAllele computer genotyping is more effective than human review

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