APh161 - Lecture 13: Molecular Motors

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Presentation transcript:

APh161 - Lecture 13: Molecular Motors Rob Phillips California Institute of Technology

Molecular Motors Mitotic Spindle Organization Cilia and Flagella Assembly and Dynamics Formation of Golgi and ER Networks Vesicle Transport

Rogues Gallery of Motor Action: Rotary Motors Show Yasuda et al. movie Show Berg movie

Rogues Gallery of Motor Action: Translocation

Rogues Gallery of Motor Action: Translational Motor 1

Rogues Gallery of Motor Action: Translational Motor 2 - Muscles See Hugh Huxley review on website

Rogues Gallery of Motor Action: Translational Motor 2 - Muscles Heuser lab – Washington University

Organellar Transport (Hirokawa, Science 1998)

Rogues Gallery of Motor Action: Translational Motor 2

Translational Motors Kinesin Myosin V Dynein Speed: 850 nm/s ATPase: 44.0 1/s Myosin V Speed: 350 nm/s ATPase: 5.0 1/s Dynein Speed: -1250 nm/s ATPase: 2.0 1/s **ALL INVOLVED IN VESICLE TRANSPORT**

How We Know: Gliding Motility Assays Show Vale movie

Dynamics of Molecular Motors Science, Vol 300, Issue 5628, 2061-2065, 27 June 2003 - Yildiz et al. Show Gelles, Selvin movie

Stepping Kinetics Spudich et al., PNAS 2000

Kinesin Data (Fisher and Kolomeisky, PNAS) Fig. 3. Kinesin velocity as a function of [ATP] under external loads, F, fixed by a force clamp. The plots, from the top down, are for F = 0, 1.05, 3.59, 4.60, and 5.63 pN, respectively. Data from Block and colleagues (9): solid curves, N = 2 fits; dashed curves, N = 2 predictions (see text)

Kinesin Data continued Fig. 4. Fits to the data of Block and colleagues (9) (and predictions) for velocity as a function of load for fixed concentrations of ATP. Note the inflection points at low [ATP] and convex profile at saturating [ATP]. (Fisher and Kolomeisky, PNAS)

Kinesin Randomness Data Fig. 5. Randomness data from Block and colleagues (9) and theoretical fits (A) as a function of external load, F, at fixed [ATP] (note that the two data points at F 5.7 pN and [ATP] = 2 mM appear separately in Block and coworkers: see figure 4 a and b of ref. 9, respectively) and (B) as a function of [ATP] at fixed loads of, from top down, F = 5.69 pN ( ), 5.35 and 4.60 pN (dashed-line predictions), 3.59 pN ( ), and 1.05 pN ( ).

What do we mean by the states?