RNA, DNA, & Proteins Chapter 12 Review

Another name for protein synthesis Main enzyme involved in linking nucleotides into DNA molecules during replication DNA polymerase Another name for protein synthesis translation Sugar used to make DNA dexoyribose

Process of making a DNA copy replication Nitrogen base with 1 ring such as cytosine and thymine pyrimidine Organism whose cells have a nucleus eukaryote

Three sequential nucleotides in an mRNA molecule that code for a Process in which the genetic code of DNA is copied into a strand of RNA transcription Three sequential nucleotides in an mRNA molecule that code for a specific amino acid codon Organism without a nucleus prokaryote

Nitrogen base with 2 rings like adenine and guanine Purine Subunit composed of a sugar, nitrogen base, and a phosphate group used to make DNA and RNA nucleotide Principle that hydrogen bonds can only form between certain nitrogen bases Base pairing

Process in which one strain of bacteria is genetically changed by receiving DNA from another transformation Series of genes that controls development and differentiation in the developing embryo Hox genes

Group of three nucleotide bases on a t-RNA molecule that are Coiled DNA and proteins that are spread out in the nucleus of non-dividing cells chromatin Group of three nucleotide bases on a t-RNA molecule that are complementary to an mRNA codon anticodon

Tightly packed DNA and proteins that are “scrunched up” into rod-shaped bundles in dividing cells chromosomes Expressed sequence of DNA that codes for a protein exon Process of making proteins from an RNA message Translation (protein synthesis)

What is CHARGAFF’S RULE? G = C Image from: http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif

Type of RNA that carries DNA code from the nucleus out to the cytoplasm Messenger RNA Segment of DNA that codes for a protein gene Region of DNA where RNA polymerase binds to start transcription promoter

Bead-like structures that form when DNA wraps around histones Protein molecule around which DNA is wrapped to form a nucleosome in chromatin histone Bonds between nitrogen bases that hold the 2 DNA strands together Hydrogen bonds Bead-like structures that form when DNA wraps around histones nucleosome

Type of RNA that matches its anticodon and attaches the correct amino acid to the growing protein chain during protein synthesis Transfer RNA Structures found in the cytoplasm made of rRNA and proteins where protein synthesis happens Ribosomes

Name the scientist who repeated Griffith’s mice-pneumonia experiment but used enzymes to destroy different kinds of molecules and showed that DNA was the molecule that caused transformation. Oswald Avery http://en.wikipedia.org/wiki/Oswald_Avery

Type of RNA that combines with proteins to make ribosomes Ribosomal RNA Sugar found in RNA molecules ribose Group of genes in a prokaryote that operate together operon

Enzyme that binds to DNA, separates Region of DNA near the promoter where repressor proteins bind and turn genes off operator Enzyme that binds to DNA, separates the strands, and assembles nucleotide subunits into an RNA molecule RNA polymerase

Protein that binds to the operator site and stops RNA polymerase from binding and transcribing a gene repressor Process of changing into different kinds of cells with different functions differentiation

Macromolecule made by joining nucleotide subunits together Nucleic acid (DNA & RNA) Virus that infects bacteria bacteriophage Another name for a protein chain polypeptide

Macromolecule made by joining amino acid subunits together protein Regulatory region with the sequence TATATA or TATAAA found in many types of organisms which helps position RNA polymerase TATA box

Sequence of DNA that is NOT involved in coding for a protein which is cut out of the m-RNA molecule before it is read by the ribosomes intron

Name the woman scientist whose X-ray images of DNA helped James Watson and Francis Crick figure out the structure of DNA ROSALYN FRANKLIN http://en.wikipedia.org/wiki/Rosalind_Franklin http://www.time.com/time/time100/scientist/profile/watsoncrick.html

Double stranded Single stranded Deoxyribose sugar Ribose sugar Tell 2 ways DNA is different from RNA DNA RNA Double stranded Single stranded Deoxyribose sugar Ribose sugar A,T,C,G A,U,C,G No Uracil No thymine Contains genetic code Carries code from Stays in nucleus nucleus to cytoplasm Helps with protein synthesis

Making a DNA copy = _____________ Using DNA code to make an RNA = ___________________ Using an RNA message to make a protein = _______________ replication transcription translation

A genetic change in a cell due to DNA received from another cell= ____________________. TRANSFORMATION

JAMES WATSON & FRANCIS CRICK Name the two scientists received the Nobel prize for figuring out the structure of DNA JAMES WATSON & FRANCIS CRICK http://en.wikipedia.org/wiki/Rosalind_Franklin http://www.time.com/time/time100/scientist/profile/watsoncrick.html

Griffith’s mice-pneumonia experiment but used enzymes to Oswald Avery repeated Griffith’s mice-pneumonia experiment but used enzymes to destroy different kinds of molecules before injecting them into the mice. Which molecule was necessary for transformation to occur? DNA http://en.wikipedia.org/wiki/Oswald_Avery

DNA → DNA = _____________ DNA → RNA = ___________________ RNA → protein = _______________ replication transcription translation

ALFRED HERSHEY & MARTHA CHASE Name the two scientists whose http://www.mun.ca/biology/scarr/Chase_&_Hershey_1953.jpg Name the two scientists whose bacteriophage-blender experiment helped show that DNA was the molecule that carried the genetic code ALFRED HERSHEY & MARTHA CHASE

A virus that infects bacteria is called a _______________ BACTERIOPHAGE http://faculty.uca.edu/~johnc/mbi1440.htm

Name this subunit used to build nucleic acids like DNA & RNA If this was going to make RNA what sugar would be used? Which nitrogen base could NOT be used? Image by: Riedell NUCLEOTIDE ribose THYMINE

Name this subunit used to build nucleic acids like DNA & RNA If this was going to make DNA what sugar would be used? Which nitrogen base could NOT be used? Image by: Riedell NUCLEOTIDE deoxyribose URACIL

Tell what nitrogen base these letters stand for _____________ = C ______________ = T ______________ = U ADENINE GUANINE CYTOSINE THYMINE URACIL

Sugar and phosphates Which molecules make up the backbone (sides of ladder) in a DNA molecule? Sugar and phosphates Image from: http://www.tokyo-med.ac.jp/genet/picts/dna.jpg

Nitrogen bases with 2 rings are called ______________ Purines G © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

DNA that is condensed in the nucleus of © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved Nucleosome Chromosome DNA double helix Coils Supercoils Histones DNA that is condensed in the nucleus of dividing cells so it can be transported easily is called _______________ CHROMOSOMES

Nitrogen bases with 1 ring are called ______________ Pyrimidines T © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

Hydrogen _____________ bonds between nitrogen bases hold the two strands together. Hydrogen Image from: http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif

PROKARYOTES different from DNA in EUKARYOTES? How is the DNA in PROKARYOTES different from DNA in EUKARYOTES? PROKARYOTES EUKARYOTES Single chromosome Many chromosomes Circular (loop) Rod-shaped bundles In cytoplasm in Nucleus (no nucleus) © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

Which protein does DNA wrap around to form nucleosomes? © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved Coils Histones HISTONES

Name the pieces of message that are NOT edited out and are expressed __________ EXONS Image by Riedell

© Pearson Education Inc, publishing as Pearson Prentice Hall © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved Chromosome DNA double helix Coils Supercoils Histones What are the small bead-like bundles called that form when DNA scrunches into chromosomes? nucleosomes

The sites where strand separation and Original strand DNA polymerase New strand Growth DNA polymerase Growth Replication fork Replication fork Nitrogenous bases New strand Original strand The sites where strand separation and replication occur are called _____________ replication forks

Name the 3 kinds of RNA RIBOSOMAL TRANSFER MESSENGER Combines with proteins to form ribosomes Matches m-RNA codon to add correct amino acids during protein synthesis carries code from DNA to ribosomes TRANSFER MESSENGER rRNA and t-RNA images from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved mRNA image from http://wps.prenhall.com/wps/media/tmp/labeling/1140654_dyn.gif

The pieces of message that are edited out are called __________ Where does this editing happen? INTRONS In nucleus Image by Riedell

Use the mRNA codon wheel to determine the amino acid being coded for: C C A =___________ C G U = __________ U U C = __________ G C A = __________ U A G = __________ proline arginine phenylalanine alanine STOP

_______________ are changes in the genetic material. MUTATIONS Tell something that can cause mutations. Mistakes in copying DNA Radiation Chemicals in environment

KINDS OF MUTATIONS Mutations that produce changes in a single gene = ______________________ Mutations that produce changes in whole chromosomes = _____________________ GENE MUTATIONS CHROMOSOMAL MUTATIONS

DNA that is spread out in the nucleus of © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved Nucleosome Chromosome DNA double helix Coils Supercoils Histones DNA that is spread out in the nucleus of non-dividing cells so the code can be read is called _______________ CHROMATIN

Mutations involving only one or a few nucleotides are called ________ mutations Explain why frameshift mutations at the beginning of a gene are more harmful than at the end. point Frameshifts cause everything after the mutation to shift and be misread so mutations at the beginning change more of the code.

Name this kind of mutation Changes one base for another A T T C G A G C T A T T C T A G C T → SUBSTITUTION

Tell the kind of mutation shown: deletion http://www.biology-online.org/2/8_mutations.htm

Name the 3 kinds of RNA RIBOSOMAL TRANSFER MESSENGER rRNA and t-RNA images from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved mRNA image from http://wps.prenhall.com/wps/media/tmp/labeling/1140654_dyn.gif

→ A T T C T A G C T Tell the kind of mutation shown: SUBSTITUTION A T T C G A G C T A T T C T A G C T → SUBSTITUTION http://www.biology-online.org/2/8_mutations.htm

Tell the kind of mutation shown: deletion http://www.biology-online.org/2/8_mutations.htm

codon Amino acid NUCLEUS m-RNA amino acid ribosome t-RNA A= _________ F= _____________ G = __________________ m-RNA amino acid ribosome t-RNA codon Amino acid Images modified from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

Tell the kind of mutation shown: INSERTION Image from: http://www.biology-online.org/2/8_mutations.htm

Mutation that causes the reading frame to shift and be misread. FRAMESHIFT MUTATION Images modified from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

Tell the kind of mutation shown: Segment flips and reads backwards INVERSION Image from: http://www.biology-online.org/2/8_mutations.htm

Tell the kind of mutation shown Segment breaks off and joins a different non-homologous chromosome TRANSLOCATION Image from: http://www.biology-online.org/2/8_mutations.htm

Condition in which an organism has extra sets of chromosomes = _______________ 3N plants are called ____________ 4N plants are called ____________ POLYPLOIDY triploid tetraploid

What advantage does polyploidy give to some plants? What do you call a polyploid human? Makes them bigger and stronger Dead. . . Humans can’t survive with polyploidy!

Group of genes that operate together are called an ________________ OPERON http://www.life.uiuc.edu/bio100/lectures/s97lects/16GeneControl/lac_operon_ind.GIF

Name the regulatory regions upstream from promoters in eukaryotic cells that bind many different regulatory proteins Which sequence in eukaryotes helps position RNA POLYMERASE? ENHANCER regions TATA box Image by Riedell

The changing of a cell to become specialized with different functions is called ___________________ How does this happen? DIFFERENTIATION http://www.ncu.edu.tw/~ls/graph/faculty_pictures/whole_time/SLC/SLC_lab-1.jpg Cells turn different genes on and off at different times

Tell what turns the lac operon off. Repressor sits on the operator blocking RNA polymerase from reading the code

What turns the lac operon ON? When lactose is present, the repressor binds lactose instead of the operator Image modified from: http://www.life.uiuc.edu/bio100/lectures/s97lects/16GeneControl/lac_operon_ind.GIF

In what kind of organisms would you find operons? Prokaryotes Eukaryotes What does the TATA box do? prokaryotes Help position RNA polymerase

Prokaryotes Eukaryotes In what kind of organisms would you find TATA boxes and enhancer regions? Prokaryotes Eukaryotes What is the function of hox genes? eukaryotes Control growth and differentiation in developing embryos