Tuesday Bell Ringer Draw and label the parts of an amino acid. How many different kinds amino acids are there? Which macromolecule is SHORT term energy? What does cellulose do? What are two amino acids linked together called? How are they linked?

P-p-p-p-p-please KARO SYRUP Wet Cleaning Wipes Eggs Vinegar Corn starch Plastic Baggies

Enzymes: “Helper” Protein molecules 2009-2010

Flow of energy through life Life is built on chemical reactions

Chemical reactions of life Processes of life building molecules synthesis breaking down molecules digestion + +

Nothing works without enzymes! How important are enzymes? all chemical reactions in living organisms require enzymes to work building molecules synthesis enzymes breaking down molecules digestive enzymes enzymes speed up reactions “catalysts” enzyme + enzyme We can’t live without enzymes! +

Examples synthesis enzyme + digestion enzyme +

Why Use Enzymes?? Enzymes lower the activation energy of reactions They make it “cheaper”

Enzymes are proteins Each enzyme is the specific helper to a specific reaction each enzyme needs to be the right shape for the job enzymes are named for the reaction they help sucrase breaks down sucrose proteases breakdown proteins lipases breakdown lipids DNA polymerase builds DNA Oh, I get it! They end in -ase

Enzymes aren’t used up Enzymes are not changed by the reaction used only temporarily re-used again for the same reaction with other molecules very little enzyme needed to help in many reactions substrate product active site enzyme

It’s shape that matters! Lock & Key model shape of protein allows enzyme & substrate to fit specific enzyme for each specific reaction

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Enzyme vocabulary Enzyme Substrate Products Active site helper protein molecule Substrate molecule that enzymes work on Products what the enzyme helps produce from the reaction Active site part of enzyme that substrate molecule fits into

What affects enzyme action Correct protein structure correct order of amino acids (Why?) Temperature Too hot or too cold = DENATURE pH (acids & bases) Does pH impact shape?

Order of amino acids Wrong order = wrong shape = can’t do its job! DNA folded protein chain of amino acids DNA right shape! folded protein chain of amino acids DNA wrong shape!

Temperature Effect on rates of enzyme activity Optimum temperature greatest number of collisions between enzyme & substrate human enzymes 35°- 40°C (body temp = 37°C) Raise temperature (boiling) denature protein = unfold = lose shape Lower temperature T° molecules move slower fewer collisions between enzyme & substrate

Temperature human enzymes reaction rate temperature 37° What’s happening here?! 37° reaction rate temperature

pH Effect on rates of enzyme activity changes in pH changes protein shape most human enzymes = pH 6-8 depends on where in body pepsin (stomach) = pH 3 trypsin (small intestines) = pH 8

pH stomach pepsin intestines trypsin reaction rate pH 1 2 3 4 5 6 7 8 What’s happening here?! reaction rate 1 2 3 4 5 6 7 8 9 10 11 12 13 14 pH

For enzymes… What matters? SHAPE! 2009-2010

Liver Lab

Building & Working Protein Using your Chrome book research a working protein. State the name of your protein. Describe its function. What is it used for? Write 4 facts about your protein.

Closure: What does the lock and key model mean? What does denature mean? What three things can denature an enzyme? What are the four functions of a protein? What process breaks apart amino acids?