HPLC Applications Training

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Presentation transcript:

HPLC Applications Training Jeff Kibby Field Support Specialist 25-March-2010

HPLC Applications Training MOBILE PHASE CONSIDERATIONS Flammability Toxicity Miscibility Viscosity Dissolved Salts %Organic Solvent pH (Acid/Alkali) Carbon Dioxide Corrosion

HPLC Applications Training Some Flammable Solvents Commonly Used Acetonitrile Methanol Hexane, iso-Hexanes, Heptane, iso-Octane Tetrahydrofuran Toluene Acetone Ethyl Acetate Isopropanol

HPLC Applications Training Some Toxic Solvents Commonly Used Acetonitrile Methanol Hexane Tetrahydrofuran Toluene Dichloromethane (Methylene Chloride) Chloroform Dimethyl Sulphoxide

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training HPLC SOLVENTS - UV CUTOFF Burdick & Jackson solvents are arranged in order of increasing UV cutoff, the wavelength at which the solvent absorbance in a 1 cm path length cell is equal to 1 AU (absorbance unit) using water in the reference cell. UV Cutoff (nm) Acetonitrile UV 190 Pentane 190 Water 190 Hexane UV 195 Cyclopentane 198 Cyclohexane 200 Heptane 200 Isopropyl Alcohol 205 Methanol 205 Ethyl Alcohol 210 2-Methoxyethanol 210 Methyl t-Butyl Ether 210 n-Propyl Alcohol 210 Trifluoroacetic Acid 210 Tetrahydrofuran UV 212 n-Butyl Alcohol 215 1,4-Dioxane 215 Ethyl Ether 215

HPLC Applications Training BURDICK & JACKSON UV Cutoff (nm) Acetonitrile UV 190 Ethylene Dichloride 228 Water Dichloromethane 233 Hexane UV 195 Chloroform 245 Cyclohexane 200 Ethyl Acetate 256 Heptane Dimethyl Acetamide 268 Isopropyl Alcohol 205 DMF Dimethyl Sulfoxide Methanol Toluene 284 Ethyl Alcohol 210 Trifluoroacetic Acid 1,2,4-TCB 308 Tetrahydrofuran UV 212 1,4-Dioxane 215 Acetone 330 Ethyl Ether Methyl Isoamyl Ketone Iso-Octane Methyl Isobutyl Ketone 334

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training HPLC Columns Silica particles (1.5~100μm) Polymer particles (3~50µm)

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training

HPLC Applications Training SAMPLE PREPARATION Filtration – Membrane Filters 0.2µm, 0.45µm (Cellulose Acetate, PVDF) Centrifugation – Economical, less contamination, multiple processing

HPLC Applications Training PS335 Photodiode Array Detector

HPLC Applications Training PS335 Photodiode Array Detector

HPLC Applications Training PS335 Photodiode Array Detector

HPLC Applications Training PS335 Photodiode Array Detector

HPLC Applications Training PS335 Photodiode Array Detector