A proteomic analysis of IVF follicular fluid in women ≤32 years old

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A proteomic analysis of IVF follicular fluid in women ≤32 years old Stephanie J. Estes, M.D., Bin Ye, Ph.D., Weiliang Qiu, Ph.D., Daniel Cramer, M.D., Mark D. Hornstein, M.D., Stacey A. Missmer, Sc.D. Fertility and Sterility Volume 92, Issue 5, Pages 1569-1578 (November 2009) DOI: 10.1016/j.fertnstert.2008.08.120 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 ELISA of the total antithrombin level in follicular fluid of success (live birth) versus failure (no pregnancy). Fertility and Sterility 2009 92, 1569-1578DOI: (10.1016/j.fertnstert.2008.08.120) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Protein/peptide profiling by two-dimensional polyacrylamide gel electrophoresis (2D PAGE). Raw data of Sypro Ruby–stained 2D PAGE images of the follicular fluid proteins from matched paired samples with live birth (top panels) and no pregnancy (bottom panels). The circled protein spots were identified visually to differ significantly between the success (live birth) group and the failure (no pregnancy) group. These spots were excised for liquid chromatography–tandem mass spectrometry analysis. Fertility and Sterility 2009 92, 1569-1578DOI: (10.1016/j.fertnstert.2008.08.120) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Protein/peptide profiling by two-dimensional gel electrophoresis (2D-PAGE). Example of spot differences in Sypro Ruby–stained 2D PAGE images of follicular fluid identified by PDQuest program comparison. (A) Spot 7101 is increased in failure (no pregnancy) versus success (live birth). (B) Spot 5402 is increased in success versus failure. The 2D gel images were reproducible, and three matched spots among all 20 gels were randomly chosen to calculate the spot position deviation. In the isoelectric focusing (IEF) direction, the spot positional deviation was 2.50 ± 0.12 mm, and in the sodium dodeclysulfate (SDS)–PAGE direction, the deviation was 0.55 ± 0.43 mm. When an outlying gel was removed from the analysis, the spot position deviations for the IEF direction and SDS-PAGE direction were 0.59 ± 0.16 mm and 0.49 ± 0.28 mm, respectively. Fertility and Sterility 2009 92, 1569-1578DOI: (10.1016/j.fertnstert.2008.08.120) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions