Quantitative Real-Time Reverse Transcription–Polymerase Chain Reaction Analysis of Drug Metabolizing and Cytoprotective Genes in Psoriasis and Regulation by Ultraviolet Radiation  Gillian Smith, Muriel M. Comrie, C. Roland Wolf  Journal of Investigative Dermatology  Volume 121, Issue 2, Pages 390-398 (August 2003) DOI: 10.1046/j.1523-1747.2003.12354.x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Constitutive cytoprotective gene expression in human skin. Quantitative real-time reverse transcription–PCR was used to investigate the constitutive expression of a variety of (A) glutathione-dependent enzymes, (B) P450, (C) drug transporters, and (D) stress response genes in nonlesional skin, taken from untreated photoprotected buttock sites of a representative panel of the patients with psoriasis (n=10), as described in Materials and Methods. Gene expression was normalized to 18S ribosomal RNA to ensure equality of loading. All samples were analyzed in triplicate. Data are presented as mean gene expression across the panel, with the exception of GSTM1 and GSTT1 where the data represent mean expression in GSTM1 (n=5) and GSTT1 (n=8) genotype-positive individuals. The standard deviation represents the extent of interindividual variation across the panel. Journal of Investigative Dermatology 2003 121, 390-398DOI: (10.1046/j.1523-1747.2003.12354.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Induction of gene expression by UVR. Changes in gene expression for: (A) COX-2; (B) GSTP1; (C) CYP1B1; (D) CYP2E1; (E) GPx-1; and (F) MRP1 in UVR irradiated skin (< 2×MED, dark blue bars; 2–3×MED, light blue bars; >3×MED, green bars) compared with control skin in the same patients are shown. Expression in PUVA-treated skin is also shown (red bars). All gene expression was normalized to 18S ribosomal RNA to ensure equality of loading and all samples were analyzed in triplicate. Each bar represents data derived from a single individual. Journal of Investigative Dermatology 2003 121, 390-398DOI: (10.1046/j.1523-1747.2003.12354.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Induction of gene expression in psoriatic plaque. Changes in gene expression for: (A) HO-1; (B) GSTP1; (C) CYP2E1; (D) MRP1; (E) CPR; and (F) GPx-1 in lesional psoriatic skin compared with control skin in the same patients are shown. All gene expression was normalized to 18S ribosomal RNA to ensure equality of loading and all samples were analyzed in triplicate. Each bar represents data derived from a single individual. Journal of Investigative Dermatology 2003 121, 390-398DOI: (10.1046/j.1523-1747.2003.12354.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions