The agony of choice in dermatophyte diagnostics—performance of different molecular tests and culture in the detection of Trichophyton rubrum and Trichophyton.

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The agony of choice in dermatophyte diagnostics—performance of different molecular tests and culture in the detection of Trichophyton rubrum and Trichophyton interdigitale C. Kupsch, T. Ohst, F. Pankewitz, P. Nenoff, S. Uhrlaß, I. Winter, Y. Gräser Clinical Microbiology and Infection Volume 22, Issue 8, Pages 735.e11-735.e17 (August 2016) DOI: 10.1016/j.cmi.2016.05.015 Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Dermatophyte positive samples detected with one or more of the four molecular methods and culture. Asterisks indicate significant differences to all other methods (p <0.001 in a Chi-square test). Clinical Microbiology and Infection 2016 22, 735.e11-735.e17DOI: (10.1016/j.cmi.2016.05.015) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Dermatophyte positive samples detected with two DNA extraction methods. In all, 375 specimens were separated into two batches: 100 samples were DNA-extracted using the QIAamp DNA Mini kit, whereas the DNA of 275 samples was isolated with the Illustra genomic DNA extraction kit and processed with qRT-PCR, ITS-ELISA and MS-ELISA. For TI-ELISA both batches of samples were DNA-extracted with the QIAamp DNA Mini kit. Clinical Microbiology and Infection 2016 22, 735.e11-735.e17DOI: (10.1016/j.cmi.2016.05.015) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions