The Role of MRD in Hematologic Malignancies

Educational Objectives

Minimal Residual Disease

Minimal Residual Disease (cont)

Flow Cytometry

MRD Sensitivity Using Flow Cytometry By Disease State

Genetic Methods for MRD Quantification

Genetic Methods for MRD Quantification (cont)

Genetic Methods for MRD Quantification (cont)

Allele-Specific Oligonucleotide (ASO)-PCR

MRD Methods – Next Generation Sequencing

MRD Methods -- Next Generation Sequencing (cont)

MRD Methods -- Next Generation Sequencing (cont)

MRD Methods -- Next Generation Sequencing (cont)

ASO-PCR vs NGS

Comparison of MRD Assessment Approaches

Current Role of MRD Assessment in Clinical Care

MRD in Acute Lymphoblastic Leukemia Children's Oncology Group AALL0232

COG AALL0232: Outcomes

ALL: Outcomes by Quantitative MRD Ranges

MRD Following HCT in ALL

Post-Transplant MRD in CLL

MRD and Outcomes in MM

MRD in Non-Hodgkin Lymphoma

Summary of MRD in Lymphoid Malignancies

Future Perspectives in MRD

Abbreviations

Abbreviations (cont)