Exploring Various Lines of Evidence for the Theory of Evolution Evidence of Evolution Exploring Various Lines of Evidence for the Theory of Evolution

Lines of Evidence DNA Sequences Comparative Anatomy Embryology Transitional Fossils Biogeography “Genetic Tool Kit”

DNA Sequences Scientists are able to isolate pieces of DNA and determine the actual sequence of nucleotides Species that are more closely related tend to have more similarities in their DNA Mice without a properly functioning leptin gene are morbidly obese (right) compared to normal mice (left). Leptin protein image

DNA Sequences Leptin = protein hormone that is important for regulating body weight and metabolism Mice without properly functioning leptin gene are morbidly obese (right) compared to normal mice (left) Leptin protein Mice without a properly functioning leptin gene are morbidly obese (right) compared to normal mice (left). Leptin protein image

DNA Sequences Compare actual sequences of DNA (leptin gene) between three different species= human, chimpanzee, mouse Predictions: how much similarity will there be? Who will be most closely related? Mice without a properly functioning leptin gene are morbidly obese (right) compared to normal mice (left). Leptin protein image

DNA Sequences First 60 nucleotides: Human: gtaggaatcg cagcgccagc ggttgcaagg taaggccccg gcgcgctcct tcctccttct Chimp: gtaggaatcg cagcgccagc ggttgcaagg taaggccccg gcgcgctcct tcctccttct Mouse: gaggga tcc ctgctccagc agctgcaagg taaggcccggggcgcgctact ttctcctcca (Mouse sequence has been shifted to line up as much as possible.) REMEMBER: Mutations can arise in the DNA sequence in a variety of forms, including nucleotide replacement, insertion, deletion, sequence inversion, etc. Mice without a properly functioning leptin gene are morbidly obese (right) compared to normal mice (left). Leptin protein image

DNA Sequences Nucleotides 121-180: Human: agtcaggagg gatgcagggc ggatggctta gttctggact atgatagctt tgtaccgagt Chimp: agtcaggagg gaggcagggc ggatggctta gttctggact atgatagctt tgtaccgagt Mouse: aggtcatgtg gacagcttgg tgttgaattc agtagttttg cagcgaggga ctctgcagac Note how the mouse sequence compares, after so many mutations have accumulated. REMEMBER: Mutations can arise in the DNA sequence in a variety of forms, including nucleotide replacement, insertion, deletion, sequence inversion, etc. (Human and Chimp sequences are identical between nucleotides 61-120.) Mice without a properly functioning leptin gene are morbidly obese (right) compared to normal mice (left). Leptin protein image

Comparative Anatomy Similarities in structures between species suggest they descended from a common ancestor. Note the color-coded bones for the limbs of these 4 mammals – though different, they share many similar bones. Describe the function of each animal’s limb on your handout.

Comparative Anatomy http://www.eskeletons.org/ Click “Comparative Anatomy” link Compare: human, chimpanzee, and squirrel monkey Predictions: Who would be the most similar and why? What similarities and differences might you expect? Follow the directions on your handout!

Embryology Ernst von Baer (1828): the more closely related any two species are, the more similar their development as embryos. In this game, you will look at pictures of embryos and guess what animal it is. Is it a snake, chicken, possum, cat, bat or human? Write your guess down on your handout, before you look at the answer!

Are you sure that’s your prediction? Embryology Are you sure that’s your prediction? Snake!

Are you sure that’s your prediction? Embryology Are you sure that’s your prediction? Bat!

Are you sure that’s your prediction? Embryology Are you sure that’s your prediction? Cat!

Are you sure that’s your prediction? Embryology Are you sure that’s your prediction? Chicken!

Are you sure that’s your prediction? Embryology Are you sure that’s your prediction? Human!

Are you sure that’s your prediction? Embryology Are you sure that’s your prediction? Possum!

Biogeography Biogeography is the study of the large-scale or global pattern of distribution of species, including the history and causes of this distribution. For this activity, you will explore the history and cause behind the distribution of marsupials. Marsupial distribution across the globe: http://evolution.berkeley.edu/evolibrary/article/0_0_0/lines_11 Distribution today split on two sides of globe – how? Review a few facts of the distribution and marsupials, as well as the history of the Earth, then formulate hypothesis behind distribution

Biogeography Marsupials are a group of mammals that give birth to live young that develop in an outer pouch of the mother. Marsupial distribution across the globe: http://evolution.berkeley.edu/evolibrary/article/0_0_0/lines_11 Distribution today split on two sides of globe – how? Review a few facts of the distribution and marsupials, as well as the history of the Earth, then formulate hypothesis behind distribution Bandicoot Koala Opossum Sugar Glider Kangaroo

Biogeography There is no evidence of any marsupials able to swim across the ocean. No marsupial has been observed wandering across the Asian mainland. There does not appear to be any route of migration between the two populations of marsupials. How do you think some marsupials ended up halfway across the world from the others?

Biogeography Continental Drift over millions of years – watch the movement of land masses

Continental Drift + Distribution of Marsupials Biogeography Continental Drift + Distribution of Marsupials

Biogeography Similar reptilian Mesosaurus fossils found in both South America and Africa  evidence of continental drift (couldn’t swim the ocean, no land bridge  continents once joined)

Biogeography Similar reptilian Mesosaurus fossils found in both South America and Africa (couldn’t swim the ocean, no land bridge)  evidence of continental drift (continents once joined)

Transitional Fossils Archaeopteryx fossils: multiple specimen found in limestone in Germany

Archaeopteryx: Berlin specimen

Archaeopteryx: Eichstatt specimen

Archaeopteryx: Solnhofen specimen

Whale Evolution Video about the transitional forms in the evolution of whales – mammals evolving from land back to sea http://www.pbs.org/wgbh/evolution/library/03/4/l_034_05.html

“Genetic Tool Kit” Video about Homeobox genes and implications for evolution: http://www.pbs.org/wgbh/evolution/library/03/4/l_034_04.html Answer the questions on your handout Image: Drosophila flies = on the left a wildtype (normal) fly, on the right a fly with the Antennapedia mutation, which causes legs to grow where the antennae should be

Eye Evolution Video about the evolution of the eye: http://www.pbs.org/wgbh/evolution/library/01/1/l_011_01.html Answer the questions on your handout