Compare the electron and light microscope

Slides:



Advertisements
Similar presentations
5 a day revision B4 - Enzymes Explain the lock and key model.
Advertisements

Chlorophyll Scientific Enquiry Dave Crowley, 2008.
Practical’s. Practical 1 Learning objectives: By the end of the lesson you should be able to State the similarities and differences between animal, plant.
Unknown Foods: Macromolecule Lab Chapter 2: Carbohydrates, Lipids, and Proteins.
We will learn The factors that affect the rate of plant growth.
4.3 (a) To investigate the effect of pH on amylase activity
AICE Biology Lab Review: Biological Molecules & Enzymes
HONORS BIOLOGY. A student performed an experiment, in which she grew germinating peas on differing water concentrations. One set of peas did not get any.
4.4 (a) To investigate the effect of temperature on amylase activity
Human Physiology ISA – ISA 1 Amylase Action
Experiment Questions Seed Digestion. State (i) An investigation in which you used starch or skimmed milk agar plates, (ii) The precise purpose for its.
Do Now  List the steps of the SCIENTIFIC METHOD.
Experiment Questions Seed Digestion. State (i) An investigation in which you used starch or skimmed milk agar plates, (ii) The precise purpose for its.
Food groups and Food tests LO: to sort food into food groups and carry out food tests TASK: 1.Why do we need to eat food and plants don’t? 2.Which food.
Food Tests D. Crowley, 2007.
The diagram below shows how food is processed in an organism.
BIO-COM QUARTERLY – STUDY GUIDE. 1. Photosynthesis takes place in the ____. CHLOROPLAST 2. What is the correct order of steps in the scientific.
Unit 1: Scientific Method. What is the scientific method? A scientific way to solve a problem. All scientists use this!
The Mysterious Tree
Starter Look at food labels Why is it useful to put the ingredients and nutritional value of food on labels? What is a food scientist? What is the Foods.
Lesson 7 How Do Plants Make Their Own Food. Brainstorm What is our evidence that plants do not get their food from the soil? If plants do not get their.
Collect a new learning booklet from the front of the classroom.
GREEN BOOK REVIEWS – STATE LAB
Learning Outcomes To re-cap on your photosynthesis knowledge from S2
Biology Chapter 1 – Cell Biology – eukaryotic and prokaryotic cells
Required Practical 6 – Using Aseptic Technique
NYSED Part D Lab Review.
Key words: Denatured, Optimum temperature, PH
4.1 (c) To conduct a qualitative test for reducing sugar
LAB: Macromolecule Virtual Lab
Starter What is the function of the digestive system?
Organisation: Lessons 2 &3 Food tests
What do we need to digest our food?
Lab #7 Osmosis in Onion Cells
Investigations with Membrane Transport
Unit 1: Scientific Method
Practical work in Biology
Using a light microscope to observe and record animal and plant cells.
Cell, Tissues & Organs i M A Explain diffusion What is a stem cell?
1.1 Cell, Tissues & Organs i M A Explain diffusion
Human digestive system
Biology Performance Event Scientific Method Review
Food Groups Tests.
Unit 1: Scientific Method
The human digestive system
5 a day revision B4 - Enzymes Explain the lock and key model.
PHOTOSYNTHESIS.
Limiting Factor How Co2 effects Plant growth and distribution.
GCSE Required Practical – Biology 1 – Using a light microscope
Topic Food tests Level GCSE (or any course for students aged 11-16)
LO: Investigate the effect of temperature on the enzyme amylase
Before you start get or make a copy of this Table of results
GCSE Required Practical – Biology 1 – Using a light microscope
Macromolecules Analysis Lab
Posing a question  Proposing a hypothesis A _______ explanation
Posing a question  Proposing a hypothesis A tentative explanation
Macromolecules Analysis Lab
PHOTOSYNTHESIS Topic 3.8 IB Biology Miss Werba.
Using a microscope Key points:
Using a light microscope to observe and record animal and plant cells.
Calorimetry Enzymes Edexcel Osmosis GCSE Biology Key Concepts Part 2
4.1 Cell Biology Lesson 10.
Required practical 5 Investigate the effect of pH on the rate of reaction of amylase.
Laboratory 1 – Food Testing
What you need to know or show
B1 content – Cell Biology (Paper 1)
Section 3.1: Biological Molecules Monomers and Polymers
Exam Skills – Method Writing
The effect of sugar or salt solutions on the mass of plant tissue
Required Practical 9: Key term What is a quadrat?
Presentation transcript:

Compare the electron and light microscope Triangle to calculate Magnification Required Practical 1 – Microscopy Use a light microscope to observe, draw and label a selection of plant and animal cells. Converting units 1cm = mm 1mm = μm 1 μm = nm Define magnification Define resolution Label the picture below Complete the table Size of image mm Magnification Actual size μm 5 X 200 15 25 X 400 10 Write a method for this practical Compare the electron and light microscope Explain the importance of using a mounted needle, cover slip and stain

What is meant by the zone of inhibition? How do you calculate it? Variables Independent: Dependent: Control: Required Practical 2 – Microbiology (GCSE Biology only) Investigate the effect of antiseptics or antibiotics on bacterial growth using agar plates and measuring zones of inhibition. What is meant by the zone of inhibition? How do you calculate it? How did you work aseptically? Why do you not seal the lid of the petri dish? Method Calculate the zone of inhibition for each antiseptic Why are the petri dishes stored upside down at 25◦C? Describe the difference between antibiotics, antiseptics and disinfectants. How could you improve the Precision: Accuracy: State and explain which antiseptic was the most effective.

Required Practical 3 – Osmosis Analysis and Conclusion Variables Independent: Dependent: Control: Required Practical 3 – Osmosis Investigate the effect of a range of concentrations of salt or sugar solutions on the mass of plant tissue. Expected Graph Definition of Osmosis Method Results   1.0 M sugar solution 0.5 M sugar solution Distilled water Initial mass (g) 10   10 Final mass in (g)  5  25 % Change in mass in How could you improve the Precision: Accuracy: Analysis and Conclusion Define the terms Hypotonic: Hypertonic: Isotonic:

Describe what is happening in the graph Variables Independent: Control: Required Practical 4 – Enzymes Investigate the effect of pH on the rate of reaction of amylase enzyme. What does amylase break down? How is the temperature controlled? Hypothesis Why do we use iodine? Explain these results Method pH 3 pH 7 pH 9 pH 12 Enzyme Key words: How could you improve the Precision: Accuracy:

What does a positive result look like? Emulsion test: Required Practical 5 – Food tests Use qualitative reagents to test for a range of carbohydrates, lipids and proteins. Risk Assessment Food tests Test for Fat: Test for Sugars: Test for Protein: Test for Starch: Hazard Risk Control Test tubes   Biuret reagent Ethanol Iodine Water bath What does a positive result look like? Emulsion test: Benedict’s reagent: Biuret reagent: Iodine test: Suggested foods to be tested Fat: Carbohydrates: Protein: A student uses Benedict’s solution to test different foods for sugar. Suggest an explanation for these results. Complete the table Food tested Final colour of Benedict’s solution A blue B brick-red C green D yellow Reagent Food group tested for Colour before adding to food Colour change if food group is present   colourless starch Benedict’s solution Purple The student says she was investigating continuous variables. Is she correct? Give your reasons. .

What is the inverse square law? - Higher Required Practical 6 – Photosynthesis Investigate the effect of a factor on the rate of photosynthesis using an aquatic organism such as pondweed. Variables Independent: Dependent: Control: What is the inverse square law? - Higher Label the diagram Expected Graph Hypothesis Method Calculate the mean and highlight any anomalies Distance between the lamp and the pondweed in cm Number of bubbles collected in minutes 1 2 3 Mean 5   35 33 37     10 30  31  15 28 27 29 20 22 23 25 18 17 30 16 11 35 7 8 What can you say about the effect of the independent variable on the dependent variable? .

Number on the ruler in cm Required Practical 7 – Reaction time Plan and carry out an investigation into the effect of a factor on human reaction time. Variables Independent: Dependent: Control: Risk Assessment and ethical considerations Other possible ways to investigate reaction times Hypothesis Method Calculate the mean and highlight any anomalies Independent variable Number on the ruler in cm 1st attempt 2nd attempt 3rd attempt 4th attempt 5th attempt Mean Silence     Music Talking After caffeine How could you improve the Precision: Accuracy: What can you say about the effect of the independent variable on the dependent variable? .

Results – Calculate the mean Required Practical 8 (GCSE Biology only) Investigate the effect of light or gravity on the growth of newly germinated seedlings. Variables Independent: Dependent: Control: Describe two ways you could extend your experiment to do further investigations. Label the diagram Figure 1 shows some cress seeds that were grown in a box with light entering on one side. Explain the scientific reason for figure 1. Hypothesis Figure 1 Results – Calculate the mean Explain why you used more than one seedling in each case. Environment Height of seedling in full sunlight in mm 1 2 3 Mean Light 25   28  27  No light  11 12  9  How could you improve the Precision: Accuracy: What can you say about the effect of the independent variable on the dependent variable? .

5,3 22 2,6 43 7,2 29 1,8 Abiotic factor investigation State the function of a quadrat Required Practical 9 – Field Investigations Measure the population size of a common species in a habitat. Use sampling techniques to investigate the effect of a factor on the distribution of this species. Risk Assessment Method Results Sample site Co-ordinates Frequency of organism Mean no of species present 5,3 22 2,6 43 7,2 29 1,8 Abiotic factor investigation Distance along transect line (m) Light intensity (lux) % of grass cover 5 1,304 35 10 1,537 40 15 1,389 20 2,224 60 25 2,114 65 30 2,423 2,455 Your quadrat has an area of 0.25 m2 Calculate the mean number of species present per m2 Explain the effect of the abiotic factor on the % of grass cover. Calculate the estimated population size . Suggest any improvements you could make to the investigation to reduce any uncertainty in your results.

Explain why the decay of milk lowers the pH Variables Independent: Dependent: Control: Required Practical 10 – Decay (GCSE Biology only) Investigate the effect of temperature on the rate of decay of fresh milk by measuring pH change. Explain why the decay of milk lowers the pH Why is lipase used in this experiment? How is the temperature controlled? Expected Graph Hypothesis Results Temperature of milk in °C Time taken for solution to turn yellow, in seconds 1 2 3 Mean 20 30 35 32 40 10 11 9 60 5 7 8 80 6 Method Two ways you could improve your experiment. What can you say about the effect of the independent variable on the dependent variable? How could you improve the Precision: Accuracy: .