Immunopathological characterization of selected mouse models of inflammatory bowel disease: Comparison to human disease  Yava L. Jones-Hall, Matthew B. Grisham  Pathophysiology  Volume 21, Issue 4, Pages 267-288 (November 2014) DOI: 10.1016/j.pathophys.2014.05.002 Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 1 Colon; IL10−/− mouse. The colon from 3-month-old mouse shows glandular elongation and distortion with mixed inflammation in the glandular lumens, lamina propria, and submucosa. Lymphocytes predominate, occasionally forming mucosal follicles. Hematoxylin and eosin (H&E) staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 2 Colon; IL10−/− mouse. The colon from a 6-month-old mouse shows a large polyp with disordered and distended glands, goblet cell hyperplasia and marked, mixed inflammation in the mucosa and submucosa. Lymphoid follicles are also seen in the lamina propria. H&E staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 3 (A).Terminal Ileum; TNF+/+ mouse at 8 weeks. The villus height and inflammatory component are normal. H&E staining. (B). Terminal Ileum; TNFΔARE/ΔARE mouse at 7 weeks. The changes are similar to those seen in the heterozygote 24-week animal. Villi are blunted and distorted, with a marked inflammatory infiltrate composed of lymphocytes, plasma cells, scattered neutrophils, and collections of submucosal histiocytes forming rudimentary granulomata. H&E staining. Reproduced with permission. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 4 Colon;. Rag1−/− mouse. This mouse was reconstituted with CD4+CD45RBlow T cells and shows no inflammation; normal goblet cells and intact epithelium; and strands of mucus on some epithelial cells. H&E staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 5 Colon; Rag1−/− mouse. This mouse was reconstituted with CD4+CD45RBhigh cells and has bowel wall thickening; transmural inflammation; loss of goblet cells; crypt abscesses; and epithelial erosion. H&E staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 6 Colon; Samp1/YitFc mouse. 10-week-old mouse ileum showed segmental inflammatory disease with loss of normal villous architecture and expansion of the lamina propria with inflammatory cells (neutrophils, lymphocytes, macrophages, and plasma cells) within areas of inflammation. The inflammatory changes are most severe in the distal portion of the ileum (center of roll) and are accompanied by thickening of the muscular wall. H&E staining. Reproduced with permission. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 7 Colon; Samp1/YitFc mouse. Higher magnification of the previous colon showing inflammation in the lamina propria and distortion of the epithelial architecture. H&E staining. Reproduced with permission. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 8 Colon; B6 mouse. This mouse was exposed to 5% DSS for 5 days. The colon is multifocally eroded with patchy areas of epithelial regeneration. There is diffuse loss of glandular architecture with mixed inflammation and necrosis in the lamina propria. The submucosa is expanded by mild edema and mixed inflammation. H&E staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 9 Colon; SV129-Smad3tm1Par/J. This mouse was chronically exposed to 3% DSS. The mucosa is markedly expanded by glandular hyperplasia and mixed inflammation. Glands are disorderly arranged, have a loss of goblet cells and are often distended by cell debris (abscess). The surface is eroded and replaced by mixed inflammation and markedly hyperplastic. There is submucosal gland herniation and marked submucosal inflammation. Herniated glands are distended by mucous and cell debris. H&E staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 10 Colon; SV129 mouse. This mouse was sensitized via skin painting followed by one IR injection of EtOH. The mouse was euthanized 3 days after IR injection. The colon shows mild, mononuclear inflammation that is confined to the mucosa. There is no erosion or loss of goblet cells. Glandular architecture is essentially normal. H&E staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions

Fig. 11 Colon; SV129 mouse. This mouse was sensitized via skin painting followed by one IR injection of TNBS/EtOH. The mouse was euthanized 3 days after IR injection. There is loss of gland with replacement by necrotic debris and mixed inflammation. The remaining glands have loss of goblet cells. There is mild expansion of the submucosa by edema and inflammation. H&E staining. Pathophysiology 2014 21, 267-288DOI: (10.1016/j.pathophys.2014.05.002) Copyright © 2014 Elsevier B.V. Terms and Conditions