Exercise 33: Effectiveness of Alcohol as an Antiseptic

Slides:

Advertisements

Similar presentations

UV light, Disinfectants, Antibiotics. UV light is used to control microbial growth Adjacent thymine molecules DNA cross link to form thymine dimer This.

Advertisements

Susceptibility of Antibacterial Chemicals on Pseudomonas aeruginosa Research performed by: Clayton Brady Health Science Biology Major Tennessee Technological.

The Effect of Three Surface Disinfectants on Four Types of Bacteria Done By: Fatimah Al Bazron Undergraduate Student (Microbiology Concentration) Department.

Antibiotic susceptibility testing Modified Kirby-Bauer method

Evaluation of antimicrobial agents and Neisseria species Lab # 7 Medgar Evers College Prof. Santos.

Chemotherapeutic and Chemical Agents Microbiology 156.

The Antibiotic Sensitivity Test Presented by Marian Mikhail Undergraduate student Biology Major Health and Science Concentration Health and Science Concentration.

Mic 224 Lab 6 Streak Plate Technique and Antibiotic Sensitivity.

Study the effect of industrial antibiotics on microbial growth.

Controlling Bacteria. Antimicrobial Agents (Bactericide) Chemical substances that either kill bacteria or inhibit bacterial growth without harming the.

Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!** Exercise 31: Lethal Effect of UV Light Score plates (+++, ++, +, -) for.

Interpretation/Evaluation of Laboratory Data CLS 552 Human Microbiology & Immunology Laboratory Note: View this PowerPoint as a ‘Full Screen Slide Show’.

Technologies + Future Outlook Microbiology. Since we have started to harness the amazing power of bacteria, the field of microbiology has become much.

Lab 13 Microbial Control with Chemical Agents. Staphylococcus aureus Escherichia coli Do all disinfectants and antiseptics work equally well against.

Exercise 41: Multiple Test Media: Read and record results

Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.

Lab 28 Goals and Objectives: Read results Exercise 31: Lethal Effect of UV Light Exercise 33: Effectiveness of Alcohol as an Antiseptic Exercise 36: Effectiveness.

Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the.

Identification of unknown bacterium

IN THE NAME OF ALLAH ALMIGHTY THE MOST COMPASSIONATE THE MERCIFUL.

Microbial DNA Synthesis Inhibitors Quinolones; fluoroquinolones Most widely used antibiotics in 2002 but their use has been recently reduced due to toxicity,

Antibiotic sensitivity

Selective and Differential Media

Urinary Tract Infection Department of Microbiology

Purell vs. Liquid Hand Soap: Purell vs. Liquid Hand Soap: which solution is more effective at killing bacteria commonly found on the surface of the hands?

Microbiology lab Urinary system. Urine culture steps 1.Collect the sample in sterile container: 1.Midstream catch. 2.Through a catheter. 3.Suprapubic.

Exercise 39: Oxidation and Fermentation Tests (Catalase)

Figure 13. The putative immunomodulatory/anti-inflammatory, anti-microbial and bactericidal mechanisms are estimated by determining the probable effective.

Antimicrobial properties of ripe Carica papaya seed.

Identification of Unknown Bacteria: individual assignment

Urinary Tract Infection Department of Microbiology

Microbial Isolation and Identification Procedures

Antimicrobial Susceptibility Testing (AST)

Identification of Unknown Bacteria: individual assignment

Antimicrobial Spectrum of Activity Visual Learning Exercises (“Flower Diagrams”) This work is licensed under the Creative Commons Attribution-NonCommercial-

Pure Culture Techniques

Pharmacology of Cephalosporins: General Overview

BIO 2071 Innovative Education--snaptutorial.com

22 –Bacteriostatic Compounds, and Antibiotic Resistance

Exercise 21: Enumeration Count colonies ( plates) and share data

Discuss formal report assignment Get acquainted with the library

Antibiotic Sensitivity

Ex. 11: Chemical Antimicrobial Agents - Antibiotics

Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli.

**NO DISINFECTING, NO HAND WASHING before Ex.33**

A new method for normalized interpretation of antimicrobial resistance from disk test results for comparative purposes G. Kronvall, G. Kahlmeter, E.

Antibiogram By:Dr. S. S. Khoramrooz In the name of God

How am I doing? Summative Tests– 1st Quarter 100% 90% 80% 70% 60% 50%

A new method for normalized interpretation of antimicrobial resistance from disk test results for comparative purposes G. Kronvall, G. Kahlmeter, E.

Trends in quinolone susceptibility of Enterobacteriaceae among inpatients of a large university hospital: 1992–98 J. Robert, E. Cambau, K. Grenet, D.

Working with the Textbook

Appropriate vs. inappropriate antimicrobial therapy

A multicentre analysis of epidemiology of the nosocomial bloodstream infections in Japanese university hospitals M. Nagao Clinical Microbiology and.

“Bug of the month “ Pseudomonas aeruginosa (Gram negative bacilli)

Biocide usage and antibiotic resistance

Multicenter study of the in vitro activity of cefepime in comparison with five other broad- spectrum antibiotics against clinical isolates of Gram-positive.

Trends in antimicrobial resistance in bloodstream infection isolates at a large urban hospital in Malawi (1998–2016): a surveillance study Patrick Musicha,

ENTEROBACTERIACEAE 1.

Antimicrobial zone of inhibition.

Antibiotic susceptibility testing

Jacques Sirot, Patrice Courvalin, Claude-James Soussy

Comparative antimicrobial spectrum and activity of the desfluoroquinolone BMS (T-3811) tested against non-fermentative Gram-negative bacilli W.

Lab# 8 Kirby-Bauer test BCH462 [practical].

Bacteria and You! Helicobacter pylori Barry Marshal.

Inhibition Zone Diameter (mm) a Gram-Negative Bacteria

Inhibition Zone Diameter (mm) a Gram-Negative Bacteria

Inhibition Zone Diameter (mm) a Gram-Negative Bacteria

Enterobacteriaceae Dr.Salma.

Pseudomonas aeruginosa 136 (15.4%) 70 (19.2%) 66 (12.7%) 0.009

Presentation transcript:

Exercise 33: Effectiveness of Alcohol as an Antiseptic Lab 27 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab Exercise 34: Antimicrobial Sensitivity Testing Measure zones of inhibition (total diameter in mm) Record millimeter diameter on class data chart Determine if organism is resistant, sensitive, or intermediate Exercise 35: Evaluation of Antiseptics Note different effects Gram + vs Gram – for some agents

Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab page 231 %reduction = (#colonies 1st) – (#colonies 2nd) (#colonies 1st) X100

Exercise 35: Evaluation of Antiseptics and Disinfectants Anything effective against only S. aureus? Only P. aeruginosa? Not effective on either? Highly effective on both? The only way to know if an agent will stop/kill a particular organism is to test it against that particular organism!

Exercise 34: Antimicrobial Sensitivity Testing Measure zones of inhibition in mm Score according to chart: S, I, R S- sensitive; is effective for this organism I- intermediate; is effective, but not the best (so why use it then?) R- resistant; is not effective for this organism Share data on class chart

Pg 237 Enterobacteriaceae = many Gram negatives: Escherichia, Proteus, Enterobacter, Klebsiella, Salmonella, Shigella…

Exercise 33: Effectiveness of Alcohol as an Antiseptic Lab 27 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab Exercise 34: Antimicrobial Sensitivity Testing Measure zones of inhibition (total diameter in mm) Record millimeter diameter on class data chart Determine if organism is resistant, sensitive, or intermediate Exercise 35: Evaluation of Antiseptics Note different effects Gram + vs Gram – for some agents