Exercise 33: Effectiveness of Alcohol as an Antiseptic

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Presentation transcript:

Exercise 33: Effectiveness of Alcohol as an Antiseptic Lab 27 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab Exercise 34: Antimicrobial Sensitivity Testing Measure zones of inhibition (total diameter in mm) Record millimeter diameter on class data chart Determine if organism is resistant, sensitive, or intermediate Exercise 35: Evaluation of Antiseptics Note different effects Gram + vs Gram – for some agents

Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab page 231 %reduction = (#colonies 1st) – (#colonies 2nd) (#colonies 1st) X100

Exercise 35: Evaluation of Antiseptics and Disinfectants Anything effective against only S. aureus? Only P. aeruginosa? Not effective on either? Highly effective on both? The only way to know if an agent will stop/kill a particular organism is to test it against that particular organism!

Exercise 34: Antimicrobial Sensitivity Testing Measure zones of inhibition in mm Score according to chart: S, I, R S- sensitive; is effective for this organism I- intermediate; is effective, but not the best (so why use it then?) R- resistant; is not effective for this organism Share data on class chart

Pg 237 Enterobacteriaceae = many Gram negatives: Escherichia, Proteus, Enterobacter, Klebsiella, Salmonella, Shigella…

Exercise 33: Effectiveness of Alcohol as an Antiseptic Lab 27 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab Exercise 34: Antimicrobial Sensitivity Testing Measure zones of inhibition (total diameter in mm) Record millimeter diameter on class data chart Determine if organism is resistant, sensitive, or intermediate Exercise 35: Evaluation of Antiseptics Note different effects Gram + vs Gram – for some agents