Chromogenic In Situ Hybridization for α6β4 Integrin in Breast Cancer

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Presentation transcript:

Chromogenic In Situ Hybridization for α6β4 Integrin in Breast Cancer Leslie K. Diaz, Xiao Zhou, Kristin Welch, Aysegul Sahin, Michael Z. Gilcrease The Journal of Molecular Diagnostics Volume 6, Issue 1, Pages 10-15 (February 2004) DOI: 10.1016/S1525-1578(10)60485-0 Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Splice variants of the β4 integrin subunit. The β4 probe recognizes a 40-nucleotide sequence in the connecting segment between the two pairs of fibronectin type III repeats in a region that does not overlap with the insertions for variants β4B and β4C. The Journal of Molecular Diagnostics 2004 6, 10-15DOI: (10.1016/S1525-1578(10)60485-0) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Immunohistochemical staining for β4 protein in breast cancer cell lines MDA-MB-231 (A) and MDA-MB-134 (B) (magnification, ×400; immunoperoxidase with DAB chromogen). In situ hybridization for β4 integrin subunit mRNA in MDA-MB-231(C) and MDA-MB-134 (D) (magnification, ×400; alkaline phosphatase with Fast Red chromogen). The Journal of Molecular Diagnostics 2004 6, 10-15DOI: (10.1016/S1525-1578(10)60485-0) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 In situ hybridization for β4 integrin subunit mRNA in a paraffin-embedded, formalin-fixed section of invasive ductal carcinoma (A), and competition with 100-fold excess of unlabeled probe (B) (magnification, ×200; alkaline phosphatase with Fast Red chromogen). The Journal of Molecular Diagnostics 2004 6, 10-15DOI: (10.1016/S1525-1578(10)60485-0) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Immunohistochemical staining for β4 protein in a frozen section of invasive ductal carcinoma (magnification, ×200; immunoperoxidase with DAB chromogen). The Journal of Molecular Diagnostics 2004 6, 10-15DOI: (10.1016/S1525-1578(10)60485-0) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions