Oral mucosa as a source of Mycobacterium leprae infection and transmission, and implications of bacterial DNA detection and the immunological status

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Oral mucosa as a source of Mycobacterium leprae infection and transmission, and implications of bacterial DNA detection and the immunological status T.S. Martinez, M.M.N.R Figueira, A.V. Costa, M.A. Gonçalves, L.R. Goulart, I.M.B. Goulart Clinical Microbiology and Infection Volume 17, Issue 11, Pages 1653-1658 (November 2011) DOI: 10.1111/j.1469-0691.2010.03453.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Detection of Mycobacterium leprae DNA by PCR. (a) Quality of the total DNA extracted in buccal swab samples from patients with leprosy visualized in agarose 0.8% gel and stained with ethidium bromide. (b) The positive detection of M. leprae DNA is demonstrated by the amplification of a 130 bp fragment of the RLEP3 genomic region: columns 1 (positive control), 3 and 6. Negative samples: columns 2, 4, 5, 7–10. B: negative control; M-100 bp marker. Fragments of 200 bp observed in columns 1–10 correspond to the amplification of the NRAMP1 gene as the internal control. Clinical Microbiology and Infection 2011 17, 1653-1658DOI: (10.1111/j.1469-0691.2010.03453.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Percentage of positivity (%) for bacterial index of skin smears and skin biopsies, Mitsuda test, serum anti-PGL1 ELISA and buccal swab in leprosy patients and their distribution according to the clinical forms of Ridley and Jopling. Clinical Microbiology and Infection 2011 17, 1653-1658DOI: (10.1111/j.1469-0691.2010.03453.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions