Do HIV+ Rapid Progressors Show More Divergence than Non-Progressors?

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Do HIV+ Rapid Progressors Show More Divergence than Non-Progressors? Karen Klyczek, Alix Darden, Susan Godfrey, Karl Beres, and Mark Bergland

Hypothesis Rapidly progressing HIV+ subjects will show more divergence in their HIV sequences between their first and last visits, compared to non-progressors.

Methods Study population: a cohort of IV drug users in Baltimore -six rapid progressors - < 200 CD4 T cells/microliter (subjects 1, 3, 4, 10, 11, 15) -three non-progressors - > 650 CD4 T cells/microliter (subjects 2, 12, 13) Generation of phylogenetic trees: 285 bp regions of the HIV-1 env gene was amplified for all subjects. Data from Markam et al. (1998) analyzed using ClustalW (Biology Workbench)

Table 1. Subject parameters for HIV study.

Progressors

Progressors Subject 3

Progressors Subject #4 Visit #1

Progressors Subject 10

Progressors Subject 11

Progressors

Nonprogressors Subject 2

Nonprogressors

Nonprogressors Subject 13

Conclusions and Discussion Overall, rapid progressors exhibited more divergence in their HIV sequences than non-progressors. In rapid progressors, sequences from clones isolated on their first visit tended to cluster separately from clones isolated on their last visit. In two of three non-progressors, there was less separation between sequences obtained from the first and last visits. More data from non-progressors are needed to confirm these differences. Reference: Markham, R.B. et al. (1998) P.N.A.S. USA 95:12568.