Surface-Engineered Gold Nanorods: Promising DNA Vaccine Adjuvant for HIV-1 Treatment Jihui Lee OCT 25, 2016.

Slides:



Advertisements
Similar presentations
“ The therapeutic effect of FIT- 06, GTU®-Multi-HIVB DNA vaccine, observed in HIV-1 infected people. Results of a Phase II trial”. Prof. Mart Ustav SVP,
Advertisements

Chapter 2 Cells.
Chapter Sixteen Science, Technology, and Global Health.
The Immune System Basics. Pathogens of Disease Bacteria –Bacteria are cellular (prokaryotic) and are Living organisms - 3 common shapes Bacilli (rod),
Treating HIV with Azidothymidine (AZT) A Design by Jeanine Nasser.
Immunoprophylaxis (prophylactic immunization). Immunoprophylaxis Types of immunization Immunoglobulins and vaccines Strategies in vaccine preparation.
NEERAJ KUMAR,AVTAR, (students) JITENDER MEHLA (Research Scholar),NDRI and Dr. S.K. Sood, Senior scientist,NDRI,Karnal.
Figure 6: Zeta potential, or surface charge, of polyplexes formed with PEI or PEI-VDP was measured by dynamic light scattering. The zeta potentials of.
Vaccines Robert Beatty MCB150. Passive vs Active Immunity  Passive immunization transfer of antibodies  Vaccines are active immunizations (mimic natural.
What living things do you see in this picture?.
Cell & Gene Therapy 2014, Las Vegas, NV October 27–29, 2014
Nanotechnology in Drug Delivery for Cancer Current Technologies
Blocviroc – an innovative treatment for HIV/AIDS Steve English Development Head, Antivirals.
NAFDAC Reg No. A7-0694L Made in the USA Manufacturer: AMERICAN JBD
Chapter 38 Viruses Objectives Define viruses Consider whether they are living or non-living Describe the structure of a virus List the names of the virus.
Types of vaccines 1 - First generation vaccines are whole-organism vaccines - either live and weakened, or killed forms. [1] Live, attenuated vaccines,
Novel strategies for prevention and treatment of HIV infection Prasit Faipenkhong Pairoaj Vonghathaipaisarn Rodjana Chunhabundit Zhang Jianjun.
General classes of vaccines An induced mobilization of the immune response for the purpose of therapeutic benefit. Preventative: infectious agents Therapeutic:
The HCV vaccine: cooperation in the shadow of the pyramids Antonella Folgori.
KEY CONCEPT Some viral diseases can be prevented with vaccines.
Combination vaccines (Presented by Dr. Shyama Saikia)
1 Biological Discovery High Volume Screening Combinatorial Diversity Structure, Design, Informatics Lead Series Biodisposition Toxicity Efficacy Pharmacokinetics.
Gene therapy and Viral Vectors Lecture 6. Contents Introduction to gene therapy Delivery of Therapeutic Genes Gene Therapy Targets Delivery Modes Steps.
SOHAIL 15-Arid-4632 PhD Scholar Department of Botany, PMAS-Arid Agriculture University, Rawalpindi.
Immunology. Announcements *There will be no lab session next week *Two weeks from today, there will be AIDS debate -three groups -work together to come.
Genetic DNA Vaccines By Chelsea Heimbrock. What are DNA Vaccines? An injection of genes of a virus into the body that creates an immune response for the.
NON-VIRAL GENE THERAPY Amanda Aryee. Gene Therapy Gene therapy is the process of introducing foreign DNA into host cells. Gene therapy has the potential.
Nanotechnology in Medicine Krešimir Pavelić, Marijeta Kralj and Damir Kralj Division of Molecular Medicine and Division of Material Chemistry Ruđer.
Reproof, Rebrand, Revalue. Executive Summary N4 Pharma - a UK-based pharma reformulation company with strong patents and proprietary technology which.
CATEGORY: VACCINES & THERAPEUTICS HIV-1 Vaccines Shokouh Makvandi-Nejad, University of Oxford, UK HIV-1 Vaccines © The copyright for this work resides.
Warm Up! What are viruses? What are some illnesses caused by viruses?
Viruses Chapter 7.1. POINT > Describe what a virus is POINT > Describe virus structure POINT > Define bacteriophage POINT > Explain how viruses reproduce.
Large animal model Equine infectious anemia virus (EIAV) Visna-maedi virus (VMV) Caprine arthritis-encephalitis virus (CAEV)
Vaccine Introduction Fahareen-Binta-Mosharraf MNS.
Theme Colours HSV Oncolytic Immunotherapy BioTrinity 2016 Lynne Braidwood.
Vaccines Stimulate immune response “safe” (non-pathogenic) 1st fish vaccine (Duff 1942) –killed bacteria preparation of A. salmonicida –1st commercial.
Vaccine; To be effective  Must stimulate as many of the body's defence mechanisms as possible.  It is not necessary to get 100% uptake of vaccine in.
Adjuvants Chemical substance that can be added to a vaccine in order to enhance the immune response to the vaccine.
Products > CFPEo (HTRpC) Transfection Reagent (Trachea Epithelium)
Ch.2 Cells Life Science.
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Viruses Chapter 7.1.
Viruses.
Endosymbiotic Theory Endosymbiotic Theory proposes that eukaryotic cells arose from living communities formed by prokaryotic organisms. Endo- means inside.
KEY CONCEPT Some viral diseases can be prevented with vaccines.
Human Health and Disease
DNA VACCINES.
US Army Medical Research and Materiel Command
Novel vaccine adjuvants for infectious diseases aninal
Chapter 2: Cells.
Objectives: Ch. 12 and 13 (host defenses)
? Neutralizing Antibodies: Research pathways in 2013 and beyond
Viral Structure, Lifecycles, HIV
Viruses.
Virus Chapter 2 Lesson 1.
Volume 15, Issue 2, Pages (February 2007)
Viruses.
Immune System Practice Test
Case presentation of non-clinical and clinical development of vaccines
I. Fluorescence Enhance
Immune System Practice Test
HIV and AIDS By: Tyra Clark.
Immune System Practice Test
Innovations in formulation and delivery systems for TLR7/8 agonists.
By JERLIN G. Research Scholar, Department of Physics.
Virus Notes.
Viruses Doesn’t belong to any kingdom -It’s not a plant or an animal.
KEY CONCEPT Some viral diseases can be prevented with vaccines.
KEY CONCEPT Some viral diseases can be prevented with vaccines.
KEY CONCEPT Some viral diseases can be prevented with vaccines.
Presentation transcript:

Surface-Engineered Gold Nanorods: Promising DNA Vaccine Adjuvant for HIV-1 Treatment Jihui Lee OCT 25, 2016

Statistics: AIDS More than 60 million people Nearly half of them died 6500 new infections daily HIV-1 Vaccine: safety and efficacy issues

DNA Vaccines Advantages: Disadvantages: Long-lived cellular immunity + humoral immunity Protective immunity Safer, inexpensive for manufacture and storage Potential for multiple antigens/pathogens Disadvantages: Immunogenecity Poor uptake DNA degradation by nuclease

Adjuvant Aluminum compounds and MF 59: Nanomaterials: AuNRs: Not active enough cellular immune response Nanomaterials: Non viral vehicles Smaller size Large surface area-to-volume ratio Ease of preparation AuNRs: Bioimaging, immunoassays, thermal therapy for tumors, drug delivery

Internalization Proton Sponge effect Cell transfection capability by CTAB- (H), PDDAC- (I), and PEI-Au NR (J) Proton Sponge effect

Internalization pEGFP: Cy3-dCTP (nick translation method) Lysosomes: LysoTrackerGreen DND-26 Mitochondria: MitoTrackerGreen FM

Internalization

Immunogenicity

Immunogenicity :

ELISPOT/ELISA

Conclusion Immunogenicity: Limitation: Enhance the uptake of DNA Activate T cell directly Limitation: Only intradermal injection PEI: Cytotoxicity, poor reproducibility

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.