J. Chen, W. Chen, M. Zhu, Y. Zhu, H. Yin, Z. Tan

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Propofol attenuates angiotensin II-induced apoptosis in human coronary artery endothelial cells J. Chen, W. Chen, M. Zhu, Y. Zhu, H. Yin, Z. Tan British Journal of Anaesthesia Volume 107, Issue 4, Pages 525-532 (October 2011) DOI: 10.1093/bja/aer197 Copyright © 2011 The Author(s) Terms and Conditions

Fig 1 The effects of propofol on Ang II-induced apoptosis in HCAECs. (a) Apoptotic cells were determined by TUNEL staining; 0.1% DMSO was used as a solvent (n=4). (b) The antiapoptotic effect of propofol on Ang II-induced apoptosis was confirmed by DNA laddering assay (representative image from four separate experiments). (c) Caspase-3 activity (n=5). Tables show the individual data points. Bars are mean and sd. British Journal of Anaesthesia 2011 107, 525-532DOI: (10.1093/bja/aer197) Copyright © 2011 The Author(s) Terms and Conditions

Fig 2 The effects of propofol on Ang II-induced NADPH oxidase expression and activity and oxidative stress in HCAECs. (a) Propofol attenuated Ang II-induced NADPH oxidase activity (n=5). (b) The effect of propofol on Ang II-induced protein expression of NADPH oxidase p22phox subunit (left), gp91phox subunit (middle), and p47phox subunit (right). GAPDH served as a loading control. The upper panel shows a representative experiment. The lower panel is the summary of densitometric data from four separate experiments. (c) Inhibitor effect of propofol on Ang II-induced oxidative stress as demonstrated by superoxide anion generation (left) and MDA (right) (n=5). Tables show the individual data points. Bars are mean and sd. British Journal of Anaesthesia 2011 107, 525-532DOI: (10.1093/bja/aer197) Copyright © 2011 The Author(s) Terms and Conditions

Fig 3 The effects of propofol on Ang II-mediated NOSIII expression, phosphorylation, and activity and NO production in HCAECs. (a) Effect of propofol on Ang II-regulated NOSIII expression (left), and phosphorylation at Thr495 (middle) and at Ser1177 (right). GAPDH served as a loading control. The upper panel is a representative experiment. The lower panel is the summary of densitometric data from five separate experiments. (b) Effect of propofol on Ang II-modulated NOSIII activity (n=5). (c) Effect of propofol and Ang II on NO production (n=5). Tables show the individual data points. Bars are mean and sd. British Journal of Anaesthesia 2011 107, 525-532DOI: (10.1093/bja/aer197) Copyright © 2011 The Author(s) Terms and Conditions

Fig 4 The effect of propofol on Ang II-activated mitochondria-dependent caspase-9 pathway. (a) Effect of propofol on Ang II-modulated caspase-3 (left), caspase-8 (middle), and caspase-9 (right) activity (n=6). (b) Effect of propofol and Ang II on the release of cytochrome c (Cyto c) from mitochondria (left), and the protein expression of Bcl-2 (middle) and C-IAP-1 (right). GAPDH served as a loading control. The upper panel is a representative experiment. The lower panel shows densitometric data (n=4). Tables show the individual data points. Bars are mean and sd. British Journal of Anaesthesia 2011 107, 525-532DOI: (10.1093/bja/aer197) Copyright © 2011 The Author(s) Terms and Conditions