SERUM CONTAINING MEDIA AND ITS COMPONENTS

INTRODUCTION MEANING OF THE TERMS: 1.MEDIA 2.SERUM

MEDIA Two types: *Natural Media *Artificial Media

NATURAL MEDIA Extracted from biological resources. CLOTS: Plasma clots NATURAL MEDIA Extracted from biological resources *CLOTS: Plasma clots *BIOLOGICAL FLUID: Amniotic fluid. Pleural fluid.aqueous humour from eye,serum *TISSUE EXTRACT: Extract from lymph,spleen,bone marrow,chick embryo extract

ARTIFICIAL MEDIA It is grouped into four classes ARTIFICIAL MEDIA It is grouped into four classes *Serum containing media *Serum free media *Chemically defined media *Protein free media

COMPOSITIONS PROTEINS: 1.FIBRONECTIN: *Attachment *spreading factors 2.TRANSFERIN: binding protein

GROWTH FACTORS: 1. PGDF: Platelets Derived Growth Factor 2 GROWTH FACTORS: 1.PGDF: Platelets Derived Growth Factor 2.EGF: Epithelial Growth factor 3.FGF:Fibroblast Growth Factor 4.IGF: Insulin like Growth Factor

GROWTH HORMONES Insulin promotes the uptake of glucose n amino acids Growth hormones may be present in serum particularly foetal serum

SERUM MEDIA ADVANTAGEs 1. complete media 2. Increases the viscosity 3 SERUM MEDIA ADVANTAGEs 1. complete media 2. Increases the viscosity 3. It acts as a buffher 4. no charged substrate is required 4.

DISADVANTAGES 1. Serum contains growth inhibitor components 2 DISADVANTAGES 1.Serum contains growth inhibitor components 2.targe variation in serum quality from one batch to other 3.it enhances the chances of contamination 4.Contains cytotoxic components.