Epicardial infarct repair with basic fibroblast growth factor–enhanced CorMatrix-ECM biomaterial attenuates postischemic cardiac remodeling Holly E.M.

Slides:

Advertisements

Similar presentations

Early effects of transcatheter aortic valve implantation and aortic valve replacement on myocardial function and aortic valve hemodynamics: Insights from.

Advertisements

Aortic valve replacement in low-flow, low-gradient aortic stenosis: Left ventricular ejection fraction matters Victor Dayan, MD, PhD, Philippe Pibarot,

T.K. Susheel Kumar, MD, Christopher J. Knott-Craig, MD

Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD,

Materials Science and Tissue Engineering: Repairing the Heart

Regional remodeling strain and its association with myocardial apoptosis after myocardial infarction in an ovine model Godfred K. Yankey, MD, Tieluo.

Fixed versus dynamic subaortic stenosis: Hemodynamics and resulting differences in Doppler echocardiography and aortic pressure contour Joon Hwa Hong,

Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.

Ventricular and pulmonary vascular remodeling induced by pulmonary overflow in a chronic model of pretricuspid shunt Daniele Linardi, MD, Alessio Rungatscher,

Cell-based gene therapy modifies matrix remodeling after a myocardial infarction in tissue inhibitor of matrix metalloproteinase-3–deficient mice Denis.

Functional evaluation of human donation after cardiac death donor hearts using a continuous isolated myocardial perfusion technique: Potential for expansion.

Mesenchymal stem cell implantation in a swine myocardial infarct model: engraftment and functional effects Jay G. Shake, MD, Peter J. Gruber, MD, PhD,

Cell transplantation preserves cardiac function after infarction by infarct stabilization: Augmentation by stem cell factor Shafie Fazel, MD, MSc, Liwen.

Ultrasound, endoscopy, and the recurrent nerve

Combined transplantation of skeletal myoblasts and angiopoietic progenitor cells reduces infarct size and apoptosis and improves cardiac function in chronic.

Potential myocardial regeneration with CorMatrix ECM: A case report

Intramyocardial thrombin promotes angiogenesis and improves cardiac function in an experimental rabbit model of acute myocardial infarction Sofoklis.

Biodegradable vs Nonbiodegradable Cardiac Support Device for Treating Ischemic Cardiomyopathy in a Canine Heart Mutsunori Kitahara, MD, Shigeru Miyagawa,

Building a bioartificial heart: A 3-song saga

A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model Qi Zhou, MD, PhD, Jian-Ye.

Myocardial regeneration for chronic heart failure: Not as easy as it sounds Richard A. Hopkins, MD The Journal of Thoracic and Cardiovascular Surgery

Heart reduction surgery: An analysis of the impact on cardiac function

Tissue-engineered pro-angiogenic fibroblast scaffold improves myocardial perfusion and function and limits ventricular remodeling after infarction J.

Intrinsic cardiac stem cells are essential for regeneration

Preventing cardiac remodeling: The combination of cell-based therapy and cardiac support therapy preserves left ventricular function in rodent model of.

Juan A. Crestanello, MD, Richard C. Daly, MD

Targeted overexpression of leukemia inhibitory factor to preserve myocardium in a rat model of postinfarction heart failure Mark F. Berry, MD, Timothy.

Levosimendan is superior to epinephrine in improving myocardial function after cardiopulmonary bypass with deep hypothermic circulatory arrest in rats

Pulmonary artery banding for idiopathic dilative cardiomyopathy: A novel therapeutic strategy using an old surgical procedure Dietmar Schranz, MD, Alex.

Intramyocardial injection of tannic acid attenuates postinfarction remodeling: A novel approach to stabilize the breaking extracellular matrix Hao Zhang,

Holly E.M. Mewhort et al. BTS 2017;2:

Improvement of cardiac function in the failing rat heart after transfer of skeletal myoblasts engineered to overexpress placental growth factor Matthias.

The engineer and the clinician: Understanding the work output and troubleshooting of the HeartMate II rotary flow pump W. Scott Arnold, MD, Kevin Bourque,

Radio frequency heating of chronic ovine infarct leads to sustained infarct area and ventricular volume reduction Mark B. Ratcliffe, MDa, Arthur W. Wallace,

Daniel Schmauss, MD, Nicolas Gerber, MSc, Ralf Sodian, MD

Novel regenerative therapy using cell-sheet covered with omentum flap delivers a huge number of cells in a porcine myocardial infarction model Yasuhiro.

The effect of pure mitral regurgitation on mitral annular geometry and three-dimensional saddle shape Tom C. Nguyen, MD, Akinobu Itoh, MD, Carl J. Carlhäll,

Inhibition of mitochondrial remodeling by cyclosporine A preserves myocardial performance in a neonatal rabbit model of cardioplegic arrest Norihiko.

In situ constructive myocardial remodeling of extracellular matrix patch enhanced with controlled growth factor release Akiko Tanaka, MD, PhD, Keigo.

Anish Krishnan, BSc, Douglas Drak, BSc, Shisan Bao, PhD, David S

Seeing a tree through the forest: Precision medicine tools can enhance donor allocation in heart transplantation Vishnu Vasanthan, MD, Paul W.M. Fedak,

Radio-guided surgery for ventricular remodeling in patients with ischemic dilated cardiomyopathy: A new tool to discriminate in vivo viable myocardium.

Rohit K. Singal, MD, MSc, FRCSC, Rakesh C. Arora, MD, PhD, FRCSC

Mitral valve repair in heart failure: Five-year follow-up from the mitral valve replacement stratum of the Acorn randomized trial Michael A. Acker, MD,

Early effects of transcatheter aortic valve implantation and aortic valve replacement on myocardial function and aortic valve hemodynamics: Insights from.

Takeyoshi Ota, MD, PhD, David Schwartzman, MD, Marco A. Zenati, MD

Acute hemodynamic effects of restrictive mitral annuloplasty in patients with end-stage heart failure: Analysis by pressure-volume relations Sven A.F.

Cellular therapy reverses myocardial dysfunction

Corticosteroids and cardiopulmonary bypass: Empiricism or science?

Clinical experience with CorMatrix extracellular matrix in the surgical treatment of mitral valve disease Marc W. Gerdisch, MD, FACS, FACC, Richard J.

Apical myectomy: A new surgical technique for management of severely symptomatic patients with apical hypertrophic cardiomyopathy Hartzell V. Schaff,

A fusion protein of hepatocyte growth factor enhances reconstruction of myocardium in a cardiac patch derived from porcine urinary bladder matrix Takeyoshi.

Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart Naosumi Sekiya, MD, Goro Matsumiya, MD, PhD, Shigeru.

Restoration of left ventricular geometry and improvement of left ventricular function in a rodent model of chronic ischemic cardiomyopathy Jiashing Yu,

Association of electrostimulation with cell transplantation in ischemic heart disease Abdel Shafy, MD, Thomas Lavergne, MD, Christian Latremouille, MD,

Replicating the success of mitral valve repair in the aortic valve

Prevascularization with gelatin microspheres containing basic fibroblast growth factor enhances the benefits of cardiomyocyte transplantation Yutaka.

Chronic septal infarction confers right ventricular protection during mechanical left ventricular unloading James Mau, BSc, MB, BS, Stuart Menzie, MB,

Himanshu J. Patel, MDa, Edward B. Lankford, MD, PhDb, David J

The Journal of Thoracic and Cardiovascular Surgery

Zhi Cui, MSc, Ren-Ke Li, MD, PhD

Innovation in cardiothoracic surgical training

Myoblast-seeded biodegradable scaffolds to prevent post–myocardial infarction evolution toward heart failure Matthias Siepe, MD, Marie-Noëlle Giraud,

The Journal of Thoracic and Cardiovascular Surgery

Discussion The Journal of Thoracic and Cardiovascular Surgery

The future of cardiac surgery training: A survival guide

Optimized ventricular restraint therapy: Adjustable restraint is superior to standard restraint in an ovine model of ischemic cardiomyopathy Lawrence.

Seeing is believing: A call for routine early postoperative hemodynamic transesophageal echocardiography monitoring after left ventricular assist device.

Patrick I. McConnell, MD, Narutoshi Hibino, MD

Right ventricular–pulmonary arterial coupling in patients after repair of tetralogy of Fallot Heiner Latus, MD, Wolfhard Binder, MD, Gunter Kerst, MD,

Presentation transcript:

Epicardial infarct repair with basic fibroblast growth factor–enhanced CorMatrix-ECM biomaterial attenuates postischemic cardiac remodeling Holly E.M. Mewhort, MD, Jeannine D. Turnbull, BSc, H. Christopher Meijndert, MBT, Janet M.C. Ngu, MD, MSc, Paul W.M. Fedak, MD, PhD, FRCSC The Journal of Thoracic and Cardiovascular Surgery Volume 147, Issue 5, Pages 1650-1659 (May 2014) DOI: 10.1016/j.jtcvs.2013.08.005 Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 A, Left ventricles (LVs) transected longitudinally through the anterior and posterior walls to reveal the infarcted myocardium within the anterior wall (indicated by the arrowheads). B, Passive pressure distension was performed in explanted LVs from normal, sham, nonenhanced CorMatrix-ECM patch (CorMatrix Cardiovascular Inc, Roswell, Ga), and basic fibroblast growth factor (bFGF)–enhanced CorMatrix-ECM patch animals (n = 5 per group). The LV volume at 30 mm Hg was recorded and compared between groups as an index of chamber size. C, Passive pressure-volume curves from normal, nonenhanced, and sham animals are shown and confirm that chamber compliance was similar between groups. ∗P < .05. ECM, Extracellular matrix. The Journal of Thoracic and Cardiovascular Surgery 2014 147, 1650-1659DOI: (10.1016/j.jtcvs.2013.08.005) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Myocardial tissue from a nonenhanced CorMatrix-ECM patch (CorMatrix Cardiovascular Inc, Roswell, Ga) animal. A, Histology. B, Immunohistochemistry. Arrowheads outline the myocardium-patch interface, green color indicates troponin, orange color indicates collagen, and blue indicates nucleus. ∗CorMatrix-ECM patch material. The Journal of Thoracic and Cardiovascular Surgery 2014 147, 1650-1659DOI: (10.1016/j.jtcvs.2013.08.005) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Immunohistochemistry. A, Normal myocardium. B, Infarcted myocardium from a sham. C, Nonenhanced CorMatrix-ECM patch (CorMatrix Cardiovascular Inc, Roswell, Ga). D, Basic fibroblast growth factor (bFGF)–enhanced CorMatrix-ECM patch animal stained with anti-collagen (orange) and 4′6-diamino2-phenylindol (blue). E, In vivo CorMatrix-ECM patch stained with anti-collagen (orange). F, In vivo CorMatrix-ECM patch stained with anti-alpha-smooth muscle actin (green) and 4′6-diamino2-phenylindol (blue). SIS ECM, Small intestine submucosa extracellular matrix. The Journal of Thoracic and Cardiovascular Surgery 2014 147, 1650-1659DOI: (10.1016/j.jtcvs.2013.08.005) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 CorMatrix-ECM patch (CorMatrix Cardiovascular Inc, Roswell, Ga) animal stained with anti-basic fibroblast growth factor (bFGF) (red) and 4′6-diamino2-phenylindol (blue). A, In vivo nonenhanced. B, bFGF-enhanced. C, CorMatrix-ECM and infarcted myocardium from a sham. D, Nonenhanced. E, bFGF-enhanced. F, Western blot for bFGF performed on the bFGF-enhanced CorMatrix-ECM patch before implantation. SIS ECM, Small intestine submucosa extracellular matrix. The Journal of Thoracic and Cardiovascular Surgery 2014 147, 1650-1659DOI: (10.1016/j.jtcvs.2013.08.005) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Hemodynamic parameters. A, Ejection fraction measured 16 weeks post-treatment by echocardiography in normal (n = 13), sham (n = 15), nonenhanced CorMatrix-ECM patch (CorMatrix Cardiovascular Inc, Roswell, Ga) (n = 18), and basic fibroblast growth factor (bFGF)–enhanced CorMatrix-ECM patch (n = 10) animals. B, Pressure-volume (PV) loop analysis in normal (n = 8), sham (n = 6), nonenhanced CorMatrix-ECM patch (n = 7), and bFGF-enhanced CorMatrix-ECM patch (n = 5) animals. C, Changes in ejection fraction measured by echocardiography over the time course of the experiment in normal (n = 13), sham (n = 15), and CorMatrix-ECM patch (n = 28; patch group includes non-enhanced and bFGF-enhanced CorMatrix-ECM patch) animals. D, Left ventricular end diastolic volume (LVEDV) measured by PV loop 16 weeks post-treatment. E, Contractility was reflected by the end systolic pressure volume relationship (ESPVR) measured by PV loop 16 weeks post-treatment. F, Ventricular compliance was reflected by the end diastolic pressure volume relationship (EDPVR) measured by PV loop 16 weeks post-treatment. ∗P < .05. ∗∗P < .01. ∗∗∗P < .001. ∗∗∗∗P < .0001. The Journal of Thoracic and Cardiovascular Surgery 2014 147, 1650-1659DOI: (10.1016/j.jtcvs.2013.08.005) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions