Dieter Steinmetz Universität Tübingen – ZMBP 18.12.15 Kurs WS 15/16 Gene Editing Dieter Steinmetz Universität Tübingen – ZMBP 18.12.15 Kurs WS 15/16
Gene Editing – 3 Main Methods ZFN „Zinc Finger Nuclease“ TALEN “Transcription Activator-Like Effector Nuclease” CRISPR/Cas9 nuclease “Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 nuclease” ZMBP - Dieter Steinmetz Quelle: 1. Baltes & Voitas 2014 2. und 3. Wikimedia Commons
ZFN „Zinc Finger Nuclease“ ZMBP - Dieter Steinmetz
ZFN – TALEN – CRISPR – gleicher Mechanismus Suchsequenz DSB Disruption oder Substitution Chen et al. GigaScience 2014 3:24 doi:10.1186/2047-217X-3-24 ZMBP
Gene targeting effektiver in CRISPR Gene targeting Experimente Merkle FT et al. (2015). Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus. Cell Reports. Volume 11, 6, 12 May 2015, Pages 875–883 http://www.sciencedirect.com/science/article/pii/S2211124715003824 ZMBP
Protein B Protein A
Recent advances enable precision in editing Random Mutagenesis & Integration RNAi Mediated Gene Knockout Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology
Recent advances enable precision in editing Random Mutagenesis & Integration RNAi Mediated Gene Knockout Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology
Random Mutagenesis & Integration RNAi Mediated Gene Knockout Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology