Supplement 1 -Psen1 -Psen2 Dasatinib (100 nM) Hours Con

Slides:

Advertisements

Similar presentations

Introduction The incidence of prostate cancer in Asians is lower compared to the Westerners in men with PSA

Advertisements

A375A375R2 Vemurafenib (µM) BRAF (Full Length) p61-BRAF Isoform pMEK pERK Tubulin Supplementary Figure S

Lyn Tyrosine Kinase Regulates Androgen Receptor Expression and Activity in Castrate Resistant Prostate Cancer Anousheh Zardan, Ka Mun Nip, Daksh Thaper,

Supplemental Figure cells/ well DLD-1 ALDH+/CD133+ DLD-1 ALDH- /CD133- Supplemental Figure 2.

親愛的吉姆舅舅：今天吃完晚餐後，奶奶說，在家裡情況變好以前，您要我搬到城裡跟您住。奶奶有沒有跟您說，爸爸已經好久沒有工作，也好久沒有人請媽媽做衣服了？我們聽完都哭了，連爸爸也哭了，但是媽媽說了一個故事讓我們又笑了。她說：您們小的時候，她曾經被您追得爬到樹上去，真的嗎？雖然我個子小，但是我很強壯，

10-3 Regulating the Cell Cycle Reading: Homework Sheet: 10-3.

Shimura Supplemental Figure 1

89 PSA recurrences over median follow-up of 11.3 years

G1 synchronous cells released at T = 0

Stem Cells & Cancer What? Where? How? Why?.

A. B. Supplemental Figure S2. A. Cell cycle G1/S Growth Factor Regulation. Publically available data from (20;

MCF-7: E2 E2(-4h)+OHT E2(-4h)+ICI (h)

Cancer: Inappropriate Expression of Stem Cell Programs?

Biological Organization

Biological Organization

Cancer Stem Cell Therapies in Gastrointestinal Cancers

GM-CSF induces tumor cell EMT and stemness.

IHC analyses of levels and/or phosphorylation status of selected proteins from representative sections of ventral prostate of Hi-Myc mice. IHC analyses.

Bortezomib 1nM PU.1 GAPDH KMS12PE U266 U937 Supplemental Figure S1.

Notch: Filling a Hole in T Helper 2 Cell Differentiation

25(OH)D and 1,25(OH)2D perfusion treatment effect on cancer-related markers: IF stains illustrating expression levels for Ki-67 (A), cyclin D1 (B), ErbB-2.

Supplemental Information

Supplemental Figure S2: G2 arrest in MCF7-shPBK1 stable cell line

Supplementary Figure 2. Effect of IL-32 and siRNA of IL-32 on melanoma, colon and prostate cancer cell growth and apoptosis as well as IL-32 expression.

SUPPLEMENTAL TABLE 1. Cell cycle profiles of HeLa cells treated

Volume 90, Issue 2, Pages (April 2016)

Supplemental Figure 5 v A Idarubicin 100 nM Idarubicin 300 nM B C D

Dasatinib, a small molecule inhibitor of the Src kinase, reduces the growth and activates apoptosis in pre-neoplastic Barrett's esophagus cell lines:

Volume 50, Issue 2, Pages (April 2006)

Sorting Out Presenilins in Alzheimer’s Disease

d a c b CD8 CD3 CD4 CD62L HLA-DR CD57 10% 85% 11% 76% 19% 60% 36% 66%

Volume 25, Issue 1, Pages (January 2014)

Volume 50, Issue 2, Pages (April 2006)

Reh cells, 24 hours c-Myc Ac-p53 b-actin

Collagen-dependent phosphorylation of eIF4E in PDAC cells.

BRAF inhibitor-resistant BRAF-mutant melanoma cells show increased invasion and metastatic potential. BRAF inhibitor-resistant BRAF-mutant melanoma cells.

Volume 14, Issue 4, Pages (October 2008)

What is the major conclusion of this paper?

Supplemental Figure 1 Cell proliferation (% of mock)

Fig. 4 High P-eIF2α expression in human prostate tumors with loss of PTEN function is associated with increased risk of metastasis or death after surgery.

Pho4 is regulated by the cyclin-dependent kinase Pho85 and responds to phosphate deprivation by translocating to nuclei. Pho4 is regulated by the cyclin-dependent.

BCR–ABL kinase activity rewires GM-CSFR signaling and confers oncogene addiction in TF1 cells. BCR–ABL kinase activity rewires GM-CSFR signaling and confers.

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

PTB-independent ShcA pools require Src to promote mammary tumorigenesis. PTB-independent ShcA pools require Src to promote mammary tumorigenesis. A, Src.

Cell viability assay and expression of COX protein.

Induction of CDCP1 is regulated by Ras in NSCLC cells.

Treatment effects of fenretinide (4-HPR, 5 μmol/L), 2-ME (2

The SFKs confer BRAF inhibitor resistance in BRAF-mutant melanoma cells. The SFKs confer BRAF inhibitor resistance in BRAF-mutant melanoma cells. A, phospho-protein.

Protein expression profile in a dasatinib-resistant cell line.

L1-expressing cells preferentially migrate into the wound edge.

AXL is not expressed in human prostate tumors.

Induction of cell death in prostate cancer cells by SAHA and ZOL

TDP1 and TOP1 protein levels vary widely in colorectal cancer (CRC) patient samples. TDP1 and TOP1 protein levels vary widely in colorectal cancer (CRC)

Expression of CRC stem cell markers and L1 in CRC cells.

ONC201 activates an ISR, decreases cyclin D1 expression, and causes an accumulation of cells in G1 in TNBC cells sensitive to the compounds' antiproliferative.

Effect of TLR-4 ligation with LPS on NF-κB activation in EOC cells: (A) MyD88+ and (B) MyD88− EOC cells were treated with 10 μg/mL LPS for 1, 2, and 4.

Functional assessment of NF-κB activation in SKBR3 cells.

Src expression in a panel of human TCC cell lines.

PTB-independent ShcA pools employ distinct domains to hyperactivate mTOR and Src signaling. PTB-independent ShcA pools employ distinct domains to hyperactivate.

BAF57 is highly expressed in human prostate cancer specimens.

VC KX-01 Total Src p-Y416 Src Supplementary Figure S1. KX-01 at low dose inhibited phosphorylation of Src in MDA-MB-231 xenografts.

Pdcd4 expression inhibits AP-1 transactivation.

AKT activation in HCC2429 is SRC- but not Notch-dependent.

Interaction of NHEJ and HR pathways in PCO cultures.

BEZ235 induces accelerated senescence after radiation (IR) in vitro.

Effect of dexamethasone on PCDGF/GP88 mRNA and protein expressions and effect of PCDGF/GP88 on dexamethasone-induced cell death. Effect of dexamethasone.

Blockade of cell cycle at G2-M phase in Bel-7402 cells treated with IG-105. Blockade of cell cycle at G2-M phase in Bel-7402 cells treated with IG-105.

Cabozantinib causes significant tumor cell elimination in vivo, but modest apoptosis induction in vitro. Cabozantinib causes significant tumor cell elimination.

Presentation transcript:

Supplement 1 -Psen1 -Psen2 Dasatinib (100 nM) Hours Con 24 48 72 -cyclin D1 -Psen1 -Psen2 - Trop2- ICD -Vinculin

A B C Supplement 2 -Psen1 -Psen2 DMSO Canertinib 200 m 24 h 48 h 40 80 120 DMSO Canertinib Canertinib Canertinib Canertinib DMSO DMSO DMSO Cell number per 104 m2 -cyclin D1 -Psen1 -Psen2 - Trop2- ICD -Vinculin

Supplement 3 Trop2 TACE ECD γ-Secretase ICD PS1 PS2 Numb Nucleus Src Cyclin D1 Stem Cell Population Numb γ-Secretase PS1 PS2 Nucleus

Human prostate cancer TMA IHC Supplement 4 Human prostate cancer TMA IHC High expression Low expression Cyclin D1 Trop-ICD Presenilin 2 Numb