Interaction of LL-37 with Model Membrane Systems of Different Complexity: Influence of the Lipid Matrix E. Sevcsik, G. Pabst, W. Richter, S. Danner,

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Presentation transcript:

Interaction of LL-37 with Model Membrane Systems of Different Complexity: Influence of the Lipid Matrix E. Sevcsik, G. Pabst, W. Richter, S. Danner, H. Amenitsch, K. Lohner Biophysical Journal Volume 94, Issue 12, Pages 4688-4699 (June 2008) DOI: 10.1529/biophysj.107.123620 Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 1 Structures of the four phospholipids used in this study. Biophysical Journal 2008 94, 4688-4699DOI: (10.1529/biophysj.107.123620) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 2 (A) Thermograms of PE/PG (I), PC/PG (II), and PC/PS (III) in the presence of 4mol % LL-37 (solid lines) recorded at a scan rate of 30°C/h. The corresponding thermograms of the pure lipid mixtures are shown in gray. Third heating scans are shown. (B) ESR spectra of a 16-DSA spin label in vesicles of PE/PG (I), PC/PG (II), and PC/PS (III) in the presence of 4mol % LL-37 (solid lines). The corresponding spectra of the pure lipid mixtures are shown in gray. T=9°C; total scan width, 100G. Biophysical Journal 2008 94, 4688-4699DOI: (10.1529/biophysj.107.123620) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 3 Contour plot of SAXS patterns of PE/PG with 4mol % LL-37 as a function of temperature. Highest intensities are colored in dark red, lowest in blue. Biophysical Journal 2008 94, 4688-4699DOI: (10.1529/biophysj.107.123620) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 4 FFTEM images of PE/PG in the presence of 4mol % LL-37. Freezing from (A) 10°C, (B) 40°C, and (C) 65°C. Scale bar, 500nm. Biophysical Journal 2008 94, 4688-4699DOI: (10.1529/biophysj.107.123620) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 5 (A) SAXS pattern of PC/PS in the presence of 4mol % LL-37 at 10°C (I) and 35°C (II) and in the absence of LL-37 at 35°C (III). Solid lines show full-q-range fits. WAXS patterns are shown in the insets. (B) FFTEM images of PC/PS in the presence of 4mol % LL-37. Freezing from 10°C (I) and 35°C (II). Scale bar, 200nm. (C) Schematic drawing of a lipid disk surrounded by LL-37 as proposed for DPPC in (14) and here for PC/PS. Biophysical Journal 2008 94, 4688-4699DOI: (10.1529/biophysj.107.123620) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 6 Schematic diagram showing the effect of LL-37 on the phospholipids DPPC, DPPE, DPPG, and DPPS and binary mixtures thereof in the gel phase (A) and in the fluid phase (B). N, no effect; B, surface binding; T, thinning; T+, major thinning (>2Å). Disk-like micelles, quasi-interdigitated phases, bilayer fragments, and disks fused to extended bilayers are shown as sketches. Biophysical Journal 2008 94, 4688-4699DOI: (10.1529/biophysj.107.123620) Copyright © 2008 The Biophysical Society Terms and Conditions