Chapter 4 Population Genetics

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Presentation transcript:

Chapter 4 Population Genetics http://learn.genetics.utah.edu/content/labs/gel/ http://learn.genetics.utah.edu/content/labs/gel/ http://learn.genetics.utah.edu/content/labs/gel/ Chapter 4 Population Genetics Diversity Within Versus Diversity Among Populations

Orchis O. isozymus O. polyzymus

O. isozymus O. polyzymus

Your Goal apply population genetics concepts to conservation planning specifically, if you have funding to save only four wetlands, which ones will it be?

Review this demonstration! http://learn.genetics.utah.edu/content/labs/gel/

Determine allele frequencies Determine frequencies of “fast” and “slow”. tally the number of alleles among the 15 individuals divide it by the total number of alleles present in the population.

Determine Allele Frequencies for Slow and Fast add the number of alleles across all 15 individuals; divide by the total # of alleles remember, homozygotes have two occurrences for Population 1 of Orchis isozymus: Slow = 23/30 = 0.77 = p Fast = 7/30 = 0.23 = q (p + q must add up to 1.0)

Wright’s Fixation Index, Fst Calculate heterozygosity = 2pq Sum heterozygosity for all three populations of each species ex. Hs for O. isozymous =  [2pq]=0.353 n 3. Calculate expected heterozygosity, Ht, if all populations were part of one, larger one Ht = 2[mean p][mean q] 4. Calculate Wright’s Fixation Index, Fst Fst = (Hs - Ht ) / Ht Fst<0.01 means little divergence Fst>0.1 means great divergence