G. Related pathways pg 117
1] CARBOHYDRATES - Cells first choice for ‘food’ or energy broken down by simple aerobic cellular respiration
2. PROTEINS H H2N—C—COOH R Proteins breakdown into amino acids deaminase removes the amino [NH2] group The remainder enters the energy cycle at various points, eg: alanine pyruvate leucine acetyl-CoA proline into Kreb’s
3. LIPIDS triglycerides glycerol + 3 fatty acids
beta oxidation [into glycolysis] glycerol 3-phosphoglycerate 3 fatty acids beta-oxidation beta oxidation most fatty acids are 18 carbons long
What does and 18-carbon fatty acid look like? H H H H H H H H H H H H H H H H H H-C-C-C-C-C-C-C-C-C-C-C-C-C-C-C-C-C-C=O H H H H H H H H H H H H H H H H H OH How many carbons in acetyl-CoA? 2 carbons How many times do we have to cut the 18 carbon fatty acid? C-C C-C C-C C-C C-C C-C C-C C-C C-C 8 cuts
To calculate the energy released by lipid breakdown, there To calculate the energy released by lipid breakdown, there are two steps. Step One: beta-oxidation step that converts a long chain of carbons into a series of acetyl-CoA The oxidation of fatty acids into acetyl-CoA molecules requires the breaking of bonds, always one less bond that the number of acetyl-CoA. To break bonds, we must add water and ATP. When these fatty acid bonds are broken, 1 FADH2 and 1 [NADH + H+] are produced.
Given these steps, the beta-oxidation of an 18 carbon fatty Given these steps, the beta-oxidation of an 18 carbon fatty acid is shown below. 18 carbon fatty acid + 9 CoASH + 8 ATP + 8 FAD + 8 NAD+ + 8 H2O 9 acetyl-CoA + 8 ( ADP + Pi ) + 8 FADH2 + 8 ( NADH + H+ )
One turn through Kreb’s cycle produces Step two: the breakdown of the acetyl-CoA through the normal Kreb’s cycle. One turn through Kreb’s cycle produces 1 ATP: 1 FADH2: 3 [NADH + H+]. To determine the total number of high energy compounds produced this way, we must multiply these base numbers by the number of acetyl-CoA’s.
1 acetyl-CoA ATP FADH2 9 acetyl-CoA ATP FADH2 # # 1 9 3 27 1 9 NADH+H+
9 acetyl-CoA ATP FADH2 # 9 27 OXIDATION NET # # -8 ATP 1 ATP 8 35 8 NADH+H+ FADH2 # 9 27 OXIDATION NET # # -8 ATP 1 ATP 8 NADH+H+ 35 NADH+H+ 8 FADH2 17 FADH2
NET ATP FADH2 # 1 35 17 NET # 3 x GLUCOSE 1 ATP 105 3 x 36 ATP 34 ATP NADH+H+ FADH2 # 1 35 17 NET # 3 x GLUCOSE 1 ATP 105 3 x 36 ATP ATP 34 ATP 140 ATP total 108 ATP
Comparing 18 carbons of fatty acid with 18 carbons of glucose 18 carbons fatty acid 140 ATP 18 carbons of glucose 108 ATP Therefore we get 140 ATP / 108 ATP or 130 % energy from the lipid compared to glucose This is why we store energy on our bodies as fat!
Now try the two examples based on 12 carbons and 20 carbons