Invasion and biofilm formation of Burkholderia dolosa is comparable with Burkholderia cenocepacia and Burkholderia multivorans Emma Caraher, Caroline.

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Invasion and biofilm formation of Burkholderia dolosa is comparable with Burkholderia cenocepacia and Burkholderia multivorans Emma Caraher, Caroline Duff, Tracy Mullen, Suzanne Mc Keon, Philip Murphy, Máire Callaghan, Siobhán McClean Journal of Cystic Fibrosis Volume 6, Issue 1, Pages 49-56 (January 2007) DOI: 10.1016/j.jcf.2006.05.007 Copyright © 2006 European Cystic Fibrosis Society Terms and Conditions

Fig. 1 Invasion of 16HBE14o-cells by B. cepacia complex strains compared to E. coli strain NCIB9485. Bars represent % invasion±standard error of three independent assays. ⁎Statistically different from control strain (P<0.05), E. coli NCIB9485. Journal of Cystic Fibrosis 2007 6, 49-56DOI: (10.1016/j.jcf.2006.05.007) Copyright © 2006 European Cystic Fibrosis Society Terms and Conditions

Fig. 2 Transmission electron micrographs of 16HBE14o-cells following two hour incubation with B. dolosa strains LMG18941 (a and b); LMG18943 (c and d); B. cenocepacia strain BC7 (e–g); E. coli strain NCIB9485 (h) or medium alone (i) at an MOI of 50:1. Both B. dolosa strains are clearly visible intracellularly (white arrows) and adjacent to the plasma membrane (dark arrows). Examples of epithelial cell projections are visible in (c). Vesicle formation was observed around BC7 cells (f, white arrow), pinching off the vesicle indicated with black arrow. Extracellular BC7 cells indicated by black arrow (e); internalised BC7 cells are indicated in (g) by white arrow. Bar=2 μm for all except (b): 1 μm and (h): 5 μm. Journal of Cystic Fibrosis 2007 6, 49-56DOI: (10.1016/j.jcf.2006.05.007) Copyright © 2006 European Cystic Fibrosis Society Terms and Conditions

Fig. 3 Biofilm formation by B. cepacia complex strains at different incubation times. Representative strains from six species were inoculated at 1×107 CFU/ml (a) or 1×106 CFU/ml (b) and grown in 96-well plates for 24 h (black) and 48 h (grey bars) and the biofilms stained with crystal violet. Bars represent the mean±standard error of three separate experiments. Journal of Cystic Fibrosis 2007 6, 49-56DOI: (10.1016/j.jcf.2006.05.007) Copyright © 2006 European Cystic Fibrosis Society Terms and Conditions