Butler Chapter 13 DC Applicants Danielle Aguirre Toni Leggett

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Presentation transcript:

Butler Chapter 13 DC Applicants Danielle Aguirre Toni Leggett Shavan Young Danielle Hoffman Travonna Carter Calvin Lee

DNA Detection Methods 1980 - Sliver staining and fluorescence techniques to detect STRs Present – Fluorescence based detection

Fluorophores

Fluorophores

Fluorescence Detection

with color contributions Normalized Fluorescent Intensity 520 540 560 580 600 620 640 WAVELENGTH (nm) 100 80 60 40 20 310 Filter Set F with color contributions 5-FAM JOE NED ROX Laser excitation (488, 514.5 nm) Normalized Fluorescent Intensity Figure 13.4, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition © 2005 Elsevier Academic Press

ABI Prism 310 Genetic Analyzer

ABI 3100 multi-capillary system

FMBIO II gel scanner

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