Do You Want to Build a Transcriptome?

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Presentation transcript:

Do You Want to Build a Transcriptome?

Transcriptome Assembly What tissue(s) How many individuals How many reads and how long Error correct or not Which Assembler? Short reads/long reads Reference guided versus de novo How to evaluate??

Transcriptome Assembly Why easier than genome assembly?

Transcriptome Assembly Why harder than genome assembly?

Transcriptome Assembly What tissues?

Transcriptome Assembly How Many Individuals??

Transcriptome Assembly How Many Reads??

Transcriptome Assembly How Many Reads??

Transcriptome Assembly

Transcriptome Assembly Error Correct??

Transcriptome Assembly Which Assembler??

Transcriptome Assembly How To Evaluate TransRate BUSCO Mapping reads Mapping transcripts

Transcriptome Assembly Short reads/long reads Reference guided versus de novo