Volume 44, Issue 1, Pages 8-16 (July 2003) DD3PCA3-based Molecular Urine Analysis for the Diagnosis of Prostate Cancer  Daphne Hessels, Jacqueline M.T. Klein Gunnewiek, Inge van Oort, Herbert F.M. Karthaus, Geert J.L. van Leenders, Bianca van Balken, Lambertus A. Kiemeney, J.Alfred Witjes, Jack A. Schalken  European Urology  Volume 44, Issue 1, Pages 8-16 (July 2003) DOI: 10.1016/S0302-2838(03)00201-X

Fig. 1 The expression of DD3/PCA3 RNA (×1·105 copies/μg tissue RNA) in non-malignant prostate (benign), prostate tissue containing equal to or less than 10% tumor cells (≤10% PCa) and prostate tissue containing more than 10% tumor cells (>10% PCa). The median expression (the thick black horizontal line in the different tissue specimen) and outliers (open circles) are shown. One extreme of 993·105 copies per microgram tissue RNA in the group of >10% PCa is not shown. European Urology 2003 44, 8-16DOI: (10.1016/S0302-2838(03)00201-X)

Fig. 2 To visualize the diagnostic efficacy of the DD3PCA3-based assay of urine samples in the absence of an arbitrary cutoff value the data were summarized using a Receiver Operating Characteristic curve (ROC). Based on this ROC curve, a cutoff level of 200·10−3 was determined (arrow). Higher cutoff values would not increase the sensitivity of this test but would only result in a loss of specificity. European Urology 2003 44, 8-16DOI: (10.1016/S0302-2838(03)00201-X)