Expression of Chemokines in GVHD Target Organs Is Influenced by Conditioning and Genetic Factors and Amplified by GVHR Markus Y. Mapara, Corinna Leng,

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Expression of Chemokines in GVHD Target Organs Is Influenced by Conditioning and Genetic Factors and Amplified by GVHR Markus Y. Mapara, Corinna Leng, Yong-Mi Kim, Roderick Bronson, Anna Lokshin, Andrew Luster, Megan Sykes Biology of Blood and Marrow Transplantation Volume 12, Issue 6, Pages 623-634 (June 2006) DOI: 10.1016/j.bbmt.2006.02.005 Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 A, Chemokine mRNA expression in the liver after syngeneic and allogeneic BMT. As described in the Methods section, BALB/c mice were lethally irradiated and reconstituted with syngeneic bone marrow alone or allogeneic B6 bone marrow plus spleen cells. Animals were killed on days 3, 5, 7, and 10. RNA was extracted and subjected to the RNAse protection assay. Each lane corresponds to 1 animal. Ten micrograms of RNA was assayed for each animal. B, Analysis of chemokine expression in the liver by RNase protection assay. Expression intensity for each band was normalized within each sample to the housekeeping gene (L32) and is presented as relative expression compared with untreated mice. Means ± SD of 2-3 animals per group are depicted at each time point. Asterisks denote significant differences (P < .05) between syngeneic and allogeneic BMT recipients at the indicated time points. Results are shown from 1 of 2 similar experiments with 2-3 animals per group at each time point. Biology of Blood and Marrow Transplantation 2006 12, 623-634DOI: (10.1016/j.bbmt.2006.02.005) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Analysis of chemokine expression in the colon by RNase protection assay. Expression intensity for each band was normalized within each sample to the housekeeping gene (L32) and is presented relative to expression in normal recipients. Means ± SD of 2-3 animals per group per time point are depicted. Asterisks denote significant differences (P < 0.05) between syngeneic and allogeneic BMT recipients at the indicated time points. Results from 1 of 2 similar experiments are shown. Biology of Blood and Marrow Transplantation 2006 12, 623-634DOI: (10.1016/j.bbmt.2006.02.005) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 Expression of CXCR3 ligand mRNA in the colon after myeloablative conditioning. As described in the Methods section, BALB/c mice were lethally irradiated and reconstituted with syngeneic bone marrow or allogeneic bone marrow plus allogeneic spleen cells (GVHD). Animals were killed on days 3, 5, 7, and 10. RNA was extracted and subjected to northern blot analysis. Blots were hybridized as described with radio-labeled cDNA probes for CXCL10, CXCL9, CXCL11, and GAPDH. Three animals per group were studied at each time point. Biology of Blood and Marrow Transplantation 2006 12, 623-634DOI: (10.1016/j.bbmt.2006.02.005) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 4 Systemic and local chemokine protein levels in response to conditioning and GVHR. A, B6 mice were lethally irradiated and reconstituted with syngeneic or allogeneic (BALB/c) BMCs. For induction of GVHD, B6 animals received 1.5 × 107 BALB/c splenocytes in addition to BALB/c BMCs. Serum was collected on days +1, +3, and +6 after BMT. Dashed line indicates the chemokine levels detected in normal B6 sera. B, Protein was extracted from colon tissue as described in the Methods section. Data from a single experiment are presented. Chemokine expression was analyzed with the Luminex assay. Data for day +3 and + 6 are shown. Means ± SD of 2-5 animals per group per time point are depicted. Asterisks denote significant differences (P < .05). The experiment was performed twice with similar results. Biology of Blood and Marrow Transplantation 2006 12, 623-634DOI: (10.1016/j.bbmt.2006.02.005) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 5 Messenger RNA expression of CXCR3 ligands in the colon after nonmyeloablative conditioning. As described in the Methods section, BALB/c or B6 mice were conditioned using the CTX-based nonmyeloablative conditioning regimen. Animals were killed on days 3, 5, 7, and 10. RNA was extracted and subjected to northern blot analysis. Blots were hybridized as described in Materials and Methods with cDNAs against CXCL10, CXCL9, CXCL11, and GAPDH. A positive control RNA sample and probe were used on each blot to ascertain successful hybridization. Three animals per group were studied at each time point. Biology of Blood and Marrow Transplantation 2006 12, 623-634DOI: (10.1016/j.bbmt.2006.02.005) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 6 Strain dependence of chemokine mRNA expression after nonmyeloablative CTX-based conditioning. Chemokine mRNA expression was analyzed in the colon by RNase protection assay. Expression intensity for each band was normalized within each sample to the housekeeping gene (L32) and is presented relative to expression in normal mice. Means ± SD of 2-3 animals per group per time point are depicted. Asterisks denote significant differences (P < .05) between CTX-conditioned B6 and BALB/c animals at the indicated time points. Biology of Blood and Marrow Transplantation 2006 12, 623-634DOI: (10.1016/j.bbmt.2006.02.005) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 7 Role of CXCR3 expression by donor cells in GVHD induction. BALB/c mice received 8-Gy TBI and were reconstituted with BMCs and splenocytes from WT (n = 18) or CXCR3−/− (n = 20) B6 mice. Recipients of CXCR3−/− splenocytes showed significantly accelerated early mortality at day 15 (P < .05, Fisher exact test). Biology of Blood and Marrow Transplantation 2006 12, 623-634DOI: (10.1016/j.bbmt.2006.02.005) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions