Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

L. Boyanova Clinical Microbiology and Infection

Gut bacterial microbiota and obesity

M.A.C. Broeren, Y. Maas, E. Retera, N.L.A. Arents

Approach to diagnosis of infective endocarditis

Clinical Microbiology and Infection

Effectiveness of Lactobacillus-containing vaginal tablets in the treatment of symptomatic bacterial vaginosis P. Mastromarino, S. Macchia, L. Meggiorini,

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

Virological tools to diagnose and monitor hepatitis C virus infection

R. Cantón Clinical Microbiology and Infection

E. Cambau, M. Drancourt Clinical Microbiology and Infection

A. Holmberg, R. Lood, M. Mörgelin, B. Söderquist, E. Holst, M

Louis Pasteur, from crystals of life to vaccination

Should standardized susceptibility testing for microbial biofilms be introduced in clinical practice? T. Coenye, D. Goeres, F. Van Bambeke, T. Bjarnsholt

Clinical Microbiology and Infection

Results from a mathematical model for human monocytic ehrlichiosis

S. Khelaifia, M. Drancourt Clinical Microbiology and Infection

Vector control: a cornerstone in the malaria elimination campaign

A. Oliver, A. Mena Clinical Microbiology and Infection

Multicenter study of the in vitro activity of cefepime in comparison with five other broad- spectrum antibiotics against clinical isolates of Gram-positive.

Training for the infectious diseases speciality in Norway

CMI editorial report 2011 Clinical Microbiology and Infection

Structural studies of lipid A from a lipopolysaccharide of the Coxiella burnetii isolate RSA 514 (Crazy) P. Vadovic, A. Fuleova, R. Ihnatko, L. Skultety,

M. Or, M. Samish, T. Waner, S. Harrus

The exclusion of dead bacterial cells is essential for accurate molecular analysis of clinical samples G.B. Rogers, P. Marsh, A.F. Stressmann, C.E. Allen,

Comparison of clinical and environmental isolates of Legionella pneumophila obtained in the UK over 19 years T.G. Harrison, N. Doshi, N.K. Fry, C.A.

Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment

Elements of design: the knowledge on which we build

In vitro selection of antibiotic resistance in Pseudomonas aeruginosa

Levofloxacin in the treatment of ventilator-associated pneumonia

Evolving epidemiology of invasive Haemophilus infections in the post-vaccination era: results from a long-term population-based study M.R. Berndsen,

Metagenomics and probiotics

Effect of antioxidants on the immune response of Helicobacter pylori

Selection of rifampicin-resistant Mycobacterium tuberculosis does not occur in the presence of low concentrations of rifaximin Ornella Soro, Adelaide.

J. Buijs, A. S. M. Dofferhoff, J. W. Mouton, J. H. T. Wagenvoort, J. W

Erythromycin susceptibility of viridans streptococci from the normal throat flora of patients treated with azithromycin or clarithromycin King A. , Bathgate.

Recent developments in staphylococcal scalded skin syndrome

Interleukin-8 production by polymorphonuclear leukocytes from patients with chronic infected leg ulcers treated with Lactobacillus plantarum M.C. Peral,

Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa R. Le Berre, S. Nguyen, E.

Update on antifungal resistance in Aspergillus and Candida

Harmonised monitoring of antimicrobial resistance in Salmonella and Campylobacter isolates from food animals in the European Union S. Bronzwaer Clinical.

Microbiological characteristics of clinical isolates of Cryptococcus neoformans in Taiwan: serotypes, mating types, molecular types, virulence factors,

Abstracts cont. Clinical Microbiology and Infection

Use of broth enrichment and real-time PCR to exclude the presence of methicillin- resistant Staphylococcus aureus in clinical samples: a sensitive screening.

Real-time PCR assays compared to culture-based approaches for identification of aerobic bacteria in chronic wounds J.H. Melendez, Y.M. Frankel, A.T.

Current experience in treating invasive zygomycosis with posaconazole

A.P. Underwood, J. Green Clinical Microbiology and Infection

Andrew M. Middleton, Maureen V. Chadwick, Harold Gaya

Increased in vitro fitness of multi- and extensively drug-resistant F15/LAM4/KZN strains of Mycobacterium tuberculosis C.C. Naidoo, M. Pillay Clinical.

H. Leblebicioglu, C. Eroglu Clinical Microbiology and Infection

Statin use and clinical outcomes among pneumonia patients

P. Kaltsas, S. Want, J. Cohen Clinical Microbiology and Infection

The influence of protein binding on the antibacterial activity of faropenem against Haemophilus influenzae I. Gustafsson, O. Cars Clinical Microbiology.

Nebulized hypertonic saline treatment in hospitalized children with moderate to severe viral bronchiolitis Z. Luo, Z. Fu, E. Liu, X. Xu, X. Fu, D. Peng,

Abstracts Clinical Microbiology and Infection

Virology: a scientific discipline facing new challenges

Modelling during an emergency: the 2009 H1N1 influenza pandemic

New oral cephalosporins in pediatric community-acquired infections

R. Khatib, G. Simeunovic, M. Sharma, M. G. Fakih, L. B. Johnson, L

Influence of climate change on the incidence and impact of arenavirus diseases: a speculative assessment J.C. Clegg Clinical Microbiology and Infection

Susceptibility of multi-drug-resistant Pseudomonas aeruginosa in intensive care units: results from the European MYSTIC study group† H. Goossens Clinical.

J.L. Balcázar Clinical Microbiology and Infection

Impact of antibiotic restrictions: the patient's perspective

B. Martha, D. Croisier, A. Fanton, K. Astruc, L. Piroth, F. Huet, P

Abstracts Clinical Microbiology and Infection

CMI readers' survey Clinical Microbiology and Infection

The future of diagnostic bacteriology

Vincent Calvez, Marc Grandadam Clinical Microbiology and Infection

Presentation transcript:

Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment J.C. Valdéz, M.C. Peral, M. Rachid, M. Santana, G. Perdigón Clinical Microbiology and Infection Volume 11, Issue 6, Pages 472-479 (June 2005) DOI: 10.1111/j.1469-0691.2005.01142.x Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Inhibitory effect of different preparations of Lactobacillus plantarum on acyl-homoserine-lactones (AHLs) produced by Pseudomonas aeruginosa as measured by β-galactosidase activity (indirect assay). AHL production by P. aeruginosa grown in Luria–Bertani (LB) medium is indicated by the dotted line. The solid line inside each box is the mean of three samples. LB, P. aeruginosa in LB medium; AF, P. aeruginosa in the presence of L. plantarum acid filtrate; NF, P. aeruginosa in the presence of L. plantarum neutralised filtrate; MRS, P. aeruginosa in MRS broth; T, P. aeruginosa in the presence of 106 CFU L. plantarum/mL. *p < 0.05; **p < 0.01; ***p < 0.001. Clinical Microbiology and Infection 2005 11, 472-479DOI: (10.1111/j.1469-0691.2005.01142.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Inhibitory effect of different preparations of Lactobacillus plantarum on the production of acyl-homoserinelactones (AHLs) by filtrates of Pseudomonas aeruginosa culture supernatant (direct assay). AHL production by filtrate of P. aeruginosa culture supernatant is indicated by the dotted line. The solid line inside each box is the mean of triplicate samples. LB, P. aeruginosa in Luria–Bertani medium; AF, P. aeruginosa in the presence of L. plantarum acid filtrate; NF, P. aeruginosa in the presence of L. plantarum neutralised filtrate; MRS, P. aeruginosa in MRS broth; T, P. aeruginosa in the presence of 106 CFU L. plantarum/mL. *p < 0.05; **p < 0.01; ***p < 0.001. Clinical Microbiology and Infection 2005 11, 472-479DOI: (10.1111/j.1469-0691.2005.01142.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Effect of different preparations of Lactobacillus plantarum on Pseudomonas aeruginosa biofilm production in static culture. Biofilm formation was monitored after incubation for 1 h and 7 h. Each point is the average of three measurements. LB, P. aeruginosa in Luria–Bertani medium; AF, P. aeruginosa in the presence of L. plantarum acid filtrate; NF, P. aeruginosa in the presence of L. plantarum neutralised filtrate; MRS, P. aeruginosa in MRS broth; T, P. aeruginosa in the presence of 106 CFU L. plantarum/mL; Lp, P. aeruginosa in the presence of L. plantarum cells washed and resuspended in phosphate-buffered saline at a concentration of 106 CFU/mL. ***p < 0.001; CI95, 95% CI for the mean. Clinical Microbiology and Infection 2005 11, 472-479DOI: (10.1111/j.1469-0691.2005.01142.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 4 Inhibitory effect of different preparations of Lactobacillus plantarum on elastase produced by Pseudomonas aeruginosa. The solid line inside each box is a mean of triplicate samples. LB, P. aeruginosa in Luria–Bertani medium; AF, P. aeruginosa in the presence of L. plantarum acid filtrate; NF, P. aeruginosa in the presence of L. plantarum neutralised filtrate; MRS, P. aeruginosa in MRS broth; T, P. aeruginosa in the presence of 106 CFU L. plantarum/mL. *p < 0.05; **p < 0.01; ***p < 0.001. Clinical Microbiology and Infection 2005 11, 472-479DOI: (10.1111/j.1469-0691.2005.01142.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 5 Comparison of Pseudomonas aeruginosa viable counts in tissue obtained from burn wounds at days 5 and 10 post-infection. Values represent the average of five independent experiments. BPs, burn wounds infected with P. aeruginosa; BPs + Lp, burn wounds infected with P. aeruginosa and treated with Lactobacillus plantarum. ***p < 0.001; CI95, 95% CI for the mean. Clinical Microbiology and Infection 2005 11, 472-479DOI: (10.1111/j.1469-0691.2005.01142.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 6 Tissue phagocytosis (TP) of Pseudomonas aeruginosa at days 5 and 10 in the burned-mouse model. Each point is the average of five experiments and each experiment was performed with cells from one mouse. BPs, burn wounds infected with P. aeruginosa; BPs + Lp, burn wounds infected with P. aeruginosa and treated with L. plantarum. ***p < 0.001; CI95, 95% CI for the mean. Clinical Microbiology and Infection 2005 11, 472-479DOI: (10.1111/j.1469-0691.2005.01142.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions