Homology 3D modeling and effect of mutations

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Presentation transcript:

Homology 3D modeling and effect of mutations Miguel Andrade Faculty of Biology, Johannes Gutenberg University Institute of Molecular Biology Mainz, Germany andrade@uni-mainz.de

Determination of protein structure X-ray crystallography (103,988 in PDB) need crystals Nuclear Magnetic Resonance (NMR) (11,212) proteins in solution lower size limit (600 aa) Electron microscopy (973) Low resolution (>5A)

Structural genomics 50% sequences covered (25% in 1995) Currently: 116K 3D structures from around 38K sequences in UniProt (how do I know?) 61M sequences in UniProt only 0.06%! 50% sequences covered (25% in 1995)

3D structure prediction Applications: target design Query sequence G K similar to L G model 3D by homology Gly Lys + catalytic center known 3D Leu Gly

3D structure prediction Applications: fit to low res 3D Query sequence 1 Query sequence 2 low resolution 3D (electron microscopy)

Domains Protein domains are structural units (average 160 aa) that share: Function Folding Evolution Proteins normally are multidomain (average 300 aa)

Domains Protein domains are structural units (average 160 aa) that share: Function Folding Evolution Proteins normally are multidomain (average 300 aa)

Domains Query Sequence Predict domains Cut No 2D Prediction Similar to PDB sequence? No 2D Prediction 3D Ab initio 3D Threading Yes 3D Modeling by homology

3D structure prediction Ab initio Explore conformational space Limit the number of atoms Break the problem into fragments of sequence Optimize hydrophobic residue burial and pairing of beta-strands Limited success

3D structure prediction Threading I-Tasser: Jeffrey Skolnick & Yang Zhang Fold 66% sequences <200 aa long of low homology to PDB Just submit your sequence and wait… (some days) Output are predicted structures (PDB format) Lee and Skolnick (2008) Biophysical Journal Roy et al (2010) Nature Methods Yang et al (2015) Nature Methods

3D structure prediction I-Tasser Roy et al (2010) Nature Methods

3D structure prediction I-Tasser http://zhanglab.ccmb.med.umich.edu/I-TASSER/

3D structure prediction I-Tasser

Aquaria Sean O’Donoghue http://aquaria.ws/ O’Donoghue et al (2015) Nature Methods

Aquaria

Aquaria

Aquaria

Exercise 1/3 Starting aquaria (May require a Java update) Works best in Firefox (in Chrome with reduced functionality) Open Firefox mit JRE (from ZDV) Go to http://aquaria.ws Run an example. If JAVA blocked unblock it at the plugin icon

Exercise 2/3 Comparing different matches in Myosin X You can load a protein by its UniProt ID Try Myosin X: http://aquaria.ws/Q9HD67/ Zoom in and out using the mouse wheel (or with shift and drag up and down). Rotate by click and drag Click on a residue to select. Shift + Click selects a range. Esc clears the selection. Double click on a residue centers the molecule on it. Left click and drag moves the molecule laterally Compare the different hits with domain annotations using the feature view Has the full structure of human myosin X being solved? Of a homolog?

Exercise 3/3 Comparing different matches in the human MR Type NR3C2 in protein name (human mineralocorticoid receptor) Note and compare the multiple hits. Which proteins are those? What do they match in the human mineralocorticoid receptor? (Use the Features view) The further down the less similar are the proteins compared. This is represented by a darker color.