Polistes species venom is devoid of carbohydrate-based cross-reactivity and allows interference-free diagnostics Simon Blank, PhD, Charles Neu, Daniel.

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Presentation transcript:

Polistes species venom is devoid of carbohydrate-based cross-reactivity and allows interference-free diagnostics Simon Blank, PhD, Charles Neu, Daniel Hasche, BSc, Frank I. Bantleon, Dipl Biol, Thilo Jakob, MD, Edzard Spillner, PhD Journal of Allergy and Clinical Immunology Volume 131, Issue 4, Pages 1239-1242 (April 2013) DOI: 10.1016/j.jaci.2012.10.047 Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Glycosylation pattern of Polistes, Apis, and Vespula venom preparations. A, AlaBlots of Apis mellifera, Polistes species, and Vespula vulgaris venom detected with Galanthus nivalis agglutinin indicating the presence of glycosylation or with anti-HRP serum specific for α1,3-core fucosylation. B, SDS-PAGE and immunoblot analysis of A mellifera, Polistes apachus, and Vespula species venom visualized as above. C, ELISA analysis of A mellifera, P apachus, and Vespula species venom and MUXF-human serum albumin conjugate by using the anti-HRP serum. GNA, Galanthus nivalis agglutinin. Journal of Allergy and Clinical Immunology 2013 131, 1239-1242DOI: (10.1016/j.jaci.2012.10.047) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Venom IgE immunoreactivity of individual CCD-reactive patient sera. A, IgE reactivity of sera of CCD-reactive HBV-allergic patients with Apis mellifera, Polistes apachus, and Vespula species venom and MUXF-HSA conjugate was assessed by using ELISA. The lower end functional cut-off is represented by a solid line. B, IgE reactivity of CCD-reactive pollen-allergic patients with MUXF, HRP, bromelain, HBV, and YJV. Polistes species venom and Polistes dominula venom as determined by ImmunoCAP. Journal of Allergy and Clinical Immunology 2013 131, 1239-1242DOI: (10.1016/j.jaci.2012.10.047) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions