CSI: Waukegan Need to Knows

Slides:

Advertisements

Similar presentations

SL Biology Unit #6 Biotech

Advertisements

Gel Electrophoresis.

Manipulating DNA Chapter 13, Section: 13 -2

Aim: How do scientists identify people using DNA Fingerprinting?

DNA Fingerprinting. We share 99.9% of our DNA with each other. That means the 0.1% of our DNA makes us unique. But that is still is over 3,000,000 differences!

Biotechnology Technology involving the DNA, genes, and, proteins of different organisms. (Chapter 9) DNA Fingerprinting w/ Gel Electrophoresis Selective.

Manipulating DNA.

Biotechnology Introduction Gel Electrophoresis. Method of analyzing DNA Allows a researcher to determine how alike or different two samples of DNA are.

Manipulation of DNA. Restriction enzymes are used to cut DNA into smaller fragments. Different restriction enzymes recognize and cut different DNA sequences.

Visualizing DNA. What is it?  Gel electrophoresis is one of the techniques scientists use to look at the DNA they have.  This technique separates DNA.

Genetics 6: Techniques for Producing and Analyzing DNA.

GEL ELECTROPHORESIS. FIRST, WHAT……  Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according.

(RFLP Electrophoresis)

Desired Gene Restriction Enzymes Bacterial defense against viral DNA Bacterial defense against viral DNA Cut DNA at specific sequences Cut DNA at specific.

DNA fingerprinting is not taking someone’s fingerprint. It is cutting up a DNA strand and separating them by size.

Gel Electrophoresis.

DNA Profiling (DNA fingerprinting) pard/cleared.html.

Science Fact of the Day: Hummingbirds can't walk..

Chapter 13-2 & 13-3: Manipulating DNA & Cell Transformation

Review Unit 1.3 Identity: Molecules and Cells. 1. What is the structure and the function of DNA? DNA Deoxyribonucleic acid – composed of nucleotides made.

3.5 GENETIC MODIFICATION AND BIOTECHNOLOGY. UNDERSTANDING Gel electrophoresis is used to separate proteins of fragments of DNA according to size PCR can.

Aim: How do scientists identify people using DNA Fingerprinting?

Biotechnology. Bell Work 1.You want to determine if a patient with leukemia has a mutation in a certain gene. What type of technology should you use and.

Gel electrophoresis.

Genetic fingerprinting

Aim: How do scientists identify people using DNA Fingerprinting?

How do scientists identify people using DNA Fingerprinting?

Selective Breeding Limitations of selective breeding and mutations:

DNA Fingerprinting (Profiling)

Copyright Pearson Prentice Hall

Aim: How do scientists identify people using DNA Fingerprinting?

Recombinant DNA Technology

Mobile Genes and Genetic Engineering Part A.

Aim: How do scientists identify people using DNA Fingerprinting?

DNA Technology & GMO Technology

DNA Fingerprinting.

DNA Fingerprinting.

DNA Analysis Target 3-2.

PCR and RLFP’s.

DNA Technology.

KEY CONCEPT DNA fingerprints (DNA testing) identify living organisms at the molecular level. (mother) (child 1) (child 2) (father)

Gel Electrophoresis Method of separating molecules within an electric field based on the size and charge of DNA fragments.

How are areas of DNA that don’t code for proteins (genes) used by our cells? How can we make use of these areas?

DNA Fingerprinting Gel Electrophoresis.

DNA Technology.

Biogenetic Engineering

Restriction Enzymes & Electrophoresis

separating & probing DNA

DNA ELECTROPHORESIS OR DNA FINGERPRINTING.

DNA Fingerprinting.

Chapter 7 DNA Fingerprinting

Title of Notes p. 9 RS DNA Fingerprinting.

Electrophoresis.

Visualizing DNA Gel Electrophoresis.

DNA Fingerprinting Gel Electrophoresis.

DNA FINGERPRINTING Gel Electrophoresis

Electrophoresis A process used to separate DNA.

Chapter 7 DNA Fingerprinting.

Genetic Engineering Terms: Plasmid

Date: January 31th, 2017 Aim #46: How do scientists identify people using DNA Fingerprinting? HW: Daily Review of Class Notes Biotechnology Textbook.

Creating a DNA Fingerprint by Gel Electrophoresis

Introduction to Gel Electrophoresis

DNA Fingerprinting.

History of DNA Fingerprinting

DNA Profiling By Derek and Blakely.

DNA FINGERPRINTING Gel Electrophoresis

DNA Technology.

Presentation transcript:

CSI: Waukegan Need to Knows Date: September 4th

DNA Typing, Profiling, Fingerprinting A process that uses DNA to show relatedness or the identity of individual humans, plants, or animals.

Importance Free innocent people suspects. Reunite children with their relatives. Identify stolen animals. Prove that whale meat has been substitute for fish in sushi. Identify remains of soldiers killed in combat. Trace diseases Determine evolutionary history.

Polymorphisms Certain non-coding regions of DNA Different in individual people. Matched to other DNA to confirm identities or similarities.

Gel Electrophoresis Process used to separate macromolecules (such as DNA sections) by size and charge. Electrical current causes the molecules to move.

Pouring Gels We will use agarose gels. It is a jelly-like substance made from seaweed. Combs are added to make the wells.

Filling the Wells We will use disposable, plastic pipettes. You must use the pipette that is labeled with your sample letter. WHY? ANSWER: If you use the same pipette, then you will contaminate your results. The results may lead to the incorrect suspect.

How do we obtain a sample? The cells are broken up to extract the DNA (just like the Cheek Cell DNA). PCR is used to create more samples of the DNA.

Sample Fragments Restriction Enzymes: cleaves (cuts) DNA into smaller segments of various sizes depending on your DNA sequence.

Why do the samples move? The gel floats in the buffer solution between the two electrodes. The electric current causes segments to move to positively-charged cathode. Why? (Hint: Think about Physics) ANSWER: DNA is negative, so it is attracted to the positive charge.

Why do the samples move? Smaller DNA segments move faster and farther than large DNA,