Gabriel Amir, MD, PhD, Xiaoyuan Ma, MD, V. Mohan Reddy, MD, Frank L

Slides:

Advertisements

Similar presentations

Uswa Shahzad, BHSc, Guangming Li, MD, Yaoguang Zhang, MD, Terrence M

Advertisements

BRCA1 Gene Mutation in Thymic Malignant Melanoma

Junya Sugiura, MD, Toshihide Nakano, MD, Hideaki Kado, MD

Impact of Cryopreservation on Extracellular Matrix Structures of Heart Valve Leaflets Katja Schenke-Layland, PhD, Navid Madershahian, MD, Iris Riemann,

Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD,

Innate Regeneration in the Aging Heart: Healing From Within

Neonatal Flail Tricuspid Valve: Diagnosis and Management

Systemic-Pulmonary Shunt Facilitates the Growth of the Pulmonary Valve Annulus in Patients With Tetralogy of Fallot Byung Kwon Chong, MD, Jae Suk Baek,

Acidosis-induced apoptosis in human and porcine heart

Rosai-Dorfman Disease of Right Atrium Mimicking Myxoma

Optimal time for cardiomyocyte transplantation to maximize myocardial function after left ventricular injury Ren-Ke Li, MD, PhD, Donald A.G. Mickle,

Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.

Marshall L. Jacobs, MD, Jeffrey P. Jacobs, MD, Sara K

Fariba Chalajour, MD, Laura A

Eva M. Marwali, MD, PhD, Cindy E

The reduction of hemodynamic loading assists self-regeneration of the injured heart by increasing cell proliferation, inhibiting cell apoptosis, and inducing.

Pediatric End-Stage Failing Hearts Demonstrate Increased Cardiac Stem Cells Brody Wehman, MD, Sudhish Sharma, PhD, Rachana Mishra, PhD, Yin Guo, PhD,

Innate Regeneration in the Aging Heart: Healing From Within

Endothelial Expression of Bone Morphogenetic Protein Receptor Type 1a is Required for Atrioventricular Valve Formation Kan Kaneko, MD, PhD, Xiaodong.

Cell transplantation preserves cardiac function after infarction by infarct stabilization: Augmentation by stem cell factor Shafie Fazel, MD, MSc, Liwen.

Volume 17, Issue 7, Pages (July 2009)

Transgenic model of cardiac rhabdomyosarcoma formation

Is the Myofibrillarlytic Myocyte a Forme Fruste Apoptotic Myocyte?

Changfa Guo, MD, Husnain Kh

Stem cell therapy in the aging hearts of Fisher 344 rats: Synergistic effects on myogenesis and angiogenesis Jiang-Yong Min, MD, Yu Chen, MD, Sohail.

Cheul Lee, MD, Soo-Jin Kim, MD, Yang Min Kim, MD

Myocardial regeneration for chronic heart failure: Not as easy as it sounds Richard A. Hopkins, MD The Journal of Thoracic and Cardiovascular Surgery

Simultaneous Removal of Right Lung Hydatid Cyst and Repair of Atrial Septal Defect in a Single Session Guang Tong, MD, PhD, Xi Lin, MD, Tao Ma, MD, PhD,

Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases Chao Xuan, MD, PhD, Ge Gao, MD, PhD, Qin Yang, MD, PhD, Xiu-Li Wang, MD, Zhi-Gang.

Transforming Growth Factor-β1 Mechanisms in Aortic Valve Calcification: Increased Alkaline Phosphatase and Related Events Jocelyn N. Clark-Greuel, PhD,

Cardiac Regeneration in the Human Left Ventricle After CorMatrix Implantation Alice Ferng, PhD, Alana Connell, MD, Martha Nunez, BS, Kitsie Johnson, BS,

Variation in Outcomes for Benchmark Operations: An Analysis of The Society of Thoracic Surgeons Congenital Heart Surgery Database Jeffrey Phillip Jacobs,

Vascular endothelial growth factor transgene expression in cell-transplanted hearts Terrence M. Yau, MD, MSc, Guangming Li, MD, Richard D Weisel, MD,

Clifford H. Van Meter, MD (by invitation), William C

Marrow Stromal Cells as Universal Donor Cells for Myocardial Regenerative Therapy: Their Unique Immune Tolerance Rony Atoui, MD, MS, Juan-Francisco Asenjo,

Endothelial nitric oxide synthase affects both early and late collateral arterial adaptation and blood flow recovery after induction of hind limb ischemia.

Vascular Endothelial Growth Factor Prevents Endothelial-to-Mesenchymal Transition in Hypertrophy Ben M.-W. Illigens, MD, Alejandra Casar Berazaluce,

Therapeutic Potential of Human Umbilical Cord Derived Stem Cells in a Rat Myocardial Infarction Model Kai Hong Wu, MD, PhD, Bin Zhou, PhD, Cun Tao Yu,

28 Years' Experience With Transatrial-Transpulmonary Repair of Atrioventricular Septal Defect With Tetralogy of Fallot Gerard J.F. Hoohenkerk, MD, Paul.

Induced Pluripotent Stem Cell Transplantation in the Treatment of Porcine Chronic Myocardial Ischemia Yifu Zhou, MD, Suna Wang, MD, Zuxi Yu, MD, PhD,

Vincent L. Gott, MD The Annals of Thoracic Surgery

Isolated Tricuspid Valve Repair After Metastatic Tumor Resection

J. Trent Magruder, MD, Todd C

Stem Cells in Thoracic Aortic Aneurysms and Dissections: Potential Contributors to Aortic Repair Ying H. Shen, MD, PhD, Xiaoqing Hu, MD, Sili Zou, MD,

Modification of Infarct Material Properties Limits Adverse Ventricular Remodeling Masato Morita, MD, Chad E. Eckert, PhD, Kanji Matsuzaki, MD, PhD, Mio.

Characterization of primary and restenotic atherosclerotic plaque from the superficial femoral artery: Potential role of Smad3 in regulation of SMC proliferation

Volume 12, Issue 6, Pages (December 2005)

Spindle Cell Sarcoma of the Mitral Valve: An Unusual Cause of Acute Coronary Syndrome in a Child Thomas Martens, MD, Kristof Vandekerckhove, MD, Katrien.

Single-Stage Resection of a Mixed Endometrial Stromal Sarcoma and Smooth Muscle Tumor With Intracardiac and Pulmonary Extension Maria Coganow, MSIV,

Surgical Results in Patients With Pulmonary Atresia-Major Aortopulmonary Collaterals in Association With Total Anomalous Pulmonary Venous Connection

Junya Sugiura, MD, Toshihide Nakano, MD, Hideaki Kado, MD

Optimal Timing for Elective Early Primary Repair of Tetralogy of Fallot: Analysis of Intermediate Term Outcomes Michael E.A. Cunningham, MD, Mary T.

Adults or Big Kids: What Is the Ideal Clinical Environment for Management of Grown-Up Patients With Congenital Heart Disease? Tara Karamlou, MD, Brian.

Surgical Repair of Pulmonary Atresia With Ventricular Septal Defect and Major Aortopulmonary Collaterals With Absent Intrapericardial Pulmonary Arteries

Surgical Correction for a Neonate With Ebstein's Anomaly Associated With Tetralogy of Fallot Ling-Yi Wei, MD, Jin-Chung Shih, MD, PhD, En-Ting Wu, MD,

Correction of Tetralogy of Fallot and of Pulmonary Atresia with Ventricular Septal Defect in Adults Jürgen Hörer, MD, Julia Friebe, MD, Christian Schreiber,

Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology.

Vascular Endothelial Growth Factor Receptor Upregulation in Response to Cell-Based Angiogenic Gene Therapy Terrence M. Yau, MD, MS, Guangming Li, MD,

Aortopulmonary Fistula After Outflow Tract Stent in Repaired Truncus

Pulmonary Valve Repair for Patients With Acquired Pulmonary Valve Insufficiency Sameh M. Said, MD, Richard D. Mainwaring, MD, Michael Ma, MD, Theresa.

Takaaki Suzuki, MD, Edward L. Bove, MD, Eric J

Peripheral blood and airway tissue expression of transforming growth factor β by neutrophils in asthmatic subjects and normal control subjects Hong Wei.

Shaun P. Setty, MD, John L. Bass, MD, K. P

Regression of Marked Myocardial Sinusoids Associated With Hypoplastic Left Heart Syndrome During Staged Fontan Approach Masamichi Ono, MD, Heidi Goerler,

Development of abnormal tissue architecture in transplanted neonatal rat myocytes Peter Whittaker, PhD, Jochen Müller-Ehmsen, MD, Joan S Dow, BS, Larry.

Mark I. Block, MD The Annals of Thoracic Surgery

Ebstein’s Anomaly of the Tricuspid Valve in Combination of Tetralogy of Fallot: Total Correction in Infancy Chao-Han Lai, MD, Jing-Ming Wu, MD, Yu-Jen.

Hilum-to-Hilum Gore-Tex Tube Replacement of Central Pulmonary Arteries

Proximal Conduit Obstruction After Sano Modified Norwood Procedure

Estimating Mortality Risk for Adult Congenital Heart Surgery: An Analysis of The Society of Thoracic Surgeons Congenital Heart Surgery Database Stephanie.

Presentation transcript:

Dynamics of Human Myocardial Progenitor Cell Populations in the Neonatal Period Gabriel Amir, MD, PhD, Xiaoyuan Ma, MD, V. Mohan Reddy, MD, Frank L. Hanley, MD, Olaf Reinhartz, MD, Chandra Ramamoorthy, MD, R. Kirk Riemer, PhD The Annals of Thoracic Surgery Volume 86, Issue 4, Pages 1311-1319 (October 2008) DOI: 10.1016/j.athoracsur.2008.06.058 Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Expression of Ki67 in human myocardium. Ki67 staining (brown) in nuclei of cells is shown in specimens from (a) fetal heart and infant heart at postnatal days (b) 4, (c) 13 (arrowheads), and (d) 22. Note the reduced density of Ki67-positive cells in neonatal myocardium with age and compared with fetal myocardium. Panel e shows a higher magnification of the area indicated by the square in Panel d to better reveal the nuclear localization of Ki67. Panel f indicates the time course of changes in Ki67 expression with postnatal age according to Norwood repair (black circles), aortopulmonary (AP) shunt (clear circles), tetralogy of Fallot (TOF, squares), truncus arteriosis (diamonds), and ventricular septal defect (VSD, plus sign). Panels g and h demonstrate coexpression of Ki67 (green) in a postnatal specimen: consecutive sections of P6 postnatal ([g] troponin T-positive or [h] sarcoplasmic reticulum calcium-regulated ATPase type 2 (SERCA2)-positive stained myocytes in red, nuclei in blue) containing Ki67-positive myocytes, indicated by the white arrowheads is shown. Panel i shows a fetal specimen section stained for the myocardial transcription factor mef2 (red), nuclei in blue, and Ki67 (green), producing a white coloration when all three dyes are colocalized (arrowheads). Figures are representative of results obtained in at least 3 specimens. Magnifications: ×400 (a to d), ×630 (g to i), ×1000 (e). Time course data for Ki67 expression, panel f: Fit of Ki67 expression vs age; linear regression R2 = 0.30; probability > |t| = 0.0030; n = 27. The Annals of Thoracic Surgery 2008 86, 1311-1319DOI: (10.1016/j.athoracsur.2008.06.058) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Expression of stage-specific embryonic antigen 4 (SSEA-4) and islet cell antigen 1 (Isl1) in human myocardium. Panels a to c show SSEA-4 staining. SSEA-4 was localized to cytoplasm and cell membrane (brown diaminobenzidine precipitate). Representative sections from (a) fetal heart near right atrium and postnatal days (b) 5 and (c) 13. Panels d to j show Isl1 staining. A region (panels d to f) of fetal heart where Isl1-positive cells were observed is located between the right ventricular outflow tract bordered by myocardium ([d] troponin T-positive staining) and adjacent to the right atrial wall ([e] atrial natriuretic peptid-positive staining). The area delineated by the rectangle in panels d to f is enlarged in panel g to reveal numerous Isl1-positive cells (some indicated by arrowheads). Panel h depicts another area adjacent to the right atrial wall of the same fetal heart, containing numerous Isl1-positive cells. Images i and j show sparse Isl1 expression (arrowheads) in postnatal day 2 and 6 myocardium. Figures are representative of results obtained in at least 3 specimens. Magnifications: a to c, g to j ×400; and d to f, ×50. The Annals of Thoracic Surgery 2008 86, 1311-1319DOI: (10.1016/j.athoracsur.2008.06.058) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Expression of c-kit in human myocardium. Representative sections from (a) fetal and neonatal myocardium at postnatal days (b) 2 and (c) 10 are shown. The brown diaminobenzidine precipitate indicates the localization of c-kit-positive cells (arrowheads), which are more numerous in fetal than postnatal myocardium but are generally few in number and localized in connective tissue adjacent to blood vessels. Panel d shows the time course for changes in c-kit expression, which declined during the first postnatal month, according to Norwood repair (black circles), aortopulmonary (AP) shunt (clear circles), tetralogy of Fallot (TOF, squares), truncus arteriosis (diamonds), and ventricular septal defect (VSD, + sign). Colocalization of c-kit with Ki67 and sarcoplasmic reticulum calcium-regulated ATPase type 2 (SERCA2) in fetal myocardium: Arrowheads in panels e and f indicate colocalization of c-kit (red) with Ki67 (green) and nucleus (blue) in (e) bright field and (f) rendered laser confocal fluorescence images. Note the presence of cells expressing each antigen alone as well as in combination: Open arrowheads indicate Ki67-positive and c-kit-negative cells; arrows indicate c-kit-positive and Ki67-negative cells. Panel g shows a confocal image of a single cell expressing both SERCA2 (green) and c-kit (red) in a field of SERCA-positive myocardium. Figures are representative of results obtained in at least 3 specimens. Magnifications: a to c, ×400; e to g, ×630. Time course data in panel d: Fit of c-kit expression vs age; linear regression R2 = 0.35; probability > |t| = 0.0013; n = 25. The Annals of Thoracic Surgery 2008 86, 1311-1319DOI: (10.1016/j.athoracsur.2008.06.058) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Expression of Nkx2.5 in human myocardium. Specimens of myocardium from postnatal day (a) 2, (b) 6, and (c) 28 hearts are shown. The brown diaminobenzidine precipitate indicates cells expressing Nkx2.5. The relative density of Nkx2.5-positive cells is generally greater in the newborn heart than in the older neonatal heart. Panel d shows the time course for changes in Nkx2.5 expression, which declined nearly threefold during the first postnatal month, according to Norwood repair (black circles), aortopulmonary (AP) shunt (clear circles), tetralogy of Fallot (TOF, squares), truncus arteriosis (diamonds), and ventricular septal defect (VSD, + sign). (Panel e) Double immunostaining analysis shows Nkx2.5 (green) with sarcoplasmic reticulum calcium-regulated ATPase type 2 (SERCA2; red) in fetal specimen. Panels f and g show Nkx2.5 (green) with (f) c-kit (red) or with (g) SERCA2 in the same P6 neonatal heart specimen. Figures are representative of results obtained in at least 3 specimens. Magnifications: a to c, ×400; e to g, ×630. Time course data for Nkx2.5 expression in Panel d: Fit of Nkx2.5 expression vs age; linear regression R2 = 0.56; probability > |t| < 0.0001; n = 26. The Annals of Thoracic Surgery 2008 86, 1311-1319DOI: (10.1016/j.athoracsur.2008.06.058) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions